Umano RPE65 Kit Elisa (A77257)

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Nome
Human RPE65 ELISA Kit
Tipo di kit
Sandwich (quantitative)
Principio di kit
This Human RPE65 ELISA Kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human RPE65 in plasma, tissue homogenates and other biological fluids.

An antibody specific for RPE65 has been coated on a microplate. Standards and samples are pipetted into the wells and the RPE65 present in each sample is bound to the wells by the immobilised antibody. The wells are washed and Anti-RPE65 Antibody (Biotin) is added. After washing away the unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted into the wells. The wells are washed again, before a TMB substrate solution is added to the wells and colour develops in proportion to the amount of RPE65 bound. The Stop Solution changes the colour from blue to yellow, and the intensity of the colour is measured at 450 nm.
Piattaforma
Pre-coated Microplate (12 x 8 well strips)
Tipo di rilevamento
Colorimetric
Instrument
Colorimetric Microplate Reader
Tipo di campione
Serum, plasma, tissue homogenates, and other biological fluids.
Sensibilità
46.875 pg/ml
Campo di rilevamento
78.125-5000 pg/ml
Tempo del kit
4h 30m
Reattività
Human
Recovery
Sample Type n Range Average
Serum 5 85% - 102% 94%
EDTA Plasma 5 89% - 101% 95%
Heparin Plasma 5 85% - 103% 95%
Linearity
Sample Type n 1:2 1:4 1:8
Serum 5 86-105% 85-104% 89-103%
EDTA Plasma 5 88-101% 86-100% 83-98%
Heparin Plasma 5 84-97% 83-95% 83-95%
Precision
Intra-assay CV: < 8%, Inter-assay CV: < 10%
Note generali
Information online is representative. Refer to the Instructions for Use inside the kit.
Conservazione
Store at +4°C. Do not use past expiration date!
Sinonimi
All-trans-retinyl-palmitate hydrolase, LCA 2, LCA2, Leber congenital amaurosis, mRPE 65, mRPE65, p63, rd 12, rd12, Retinal pigment epithelium specific 61 kDa protein, Retinal pigment epithelium specific 65 kDa protein, Retinal pigment epithelium specific protein, Retinal pigment epithelium specific protein 65kDa, Retinal pigment epithelium-specific 65 kDa protein, Retinitis pigmentosa 20, Retinoid isomerohydrolase, Retinol isomerase, RP 20, RP20, RPE 65, RPE65_HUMAN, sRPE 65, sRPE65
Dichiarazione di non responsabilità
Questo prodotto è solo per uso di ricerca. Non è inteso per uso diagnostico o terapeutico.
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