Human TRAIL ELISA Kit is based on the quantitative sandwich enzyme-linked immunosorbent assay technique to measure concentration of human TRAIL in the samples. A monoclonal antibody specific for human TRAIL has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and TRAIL present is bound by the immobilized antibody. After washing away any unbound substances, a biotin-linked detect antibody specific for TRAIL is added to the wells. Following a wash to remove any unbound antibody-biotin reagent, streptavidin-HRP is added. After washing, substrate solution is added to the wells and color develops in proportion to the amount of TRAIL bound in the initial step. The color development is stopped and the intensity of the color is measured.

Piattaforma

Microplate

Tipo di rilevamento

Colorimetric

Tipo di campione

Cell culture supernatant, serum and plasma.

Sensibilità

8.19 pg/ml

Campo di rilevamento

62.50 - 4000 pg/ml

Recupero

97% (Average Recovery from Serum Samples).

Specificità

This kit can assay both natural and recombinant human TRAIL.

Reattività

Human

Reattività incrociata

This assay has shown no significant cross-reactivity.

Precision

Intra-assay CV: < 8%, Inter-assay CV: < 10%

Conservazione

Store at 2 - 8°C.

Sinonimi

Apo 2 ligand, APO 2L, Apo-2 ligand, Apo-2L, APO2L, CD253, CD253 antigen, Chemokine tumor necrosis factor ligand superfamily member 10, Protein TRAIL, TL2, TNF Related Apoptosis Inducing Ligand, TNF related apoptosis inducing ligand TRAIL, TNF-related apoptosis-inducing ligand, TNF10_HUMAN, TNFSF10, Tumor necrosis factor (ligand) family member 10, Tumor Necrosis Factor (ligand) Superfamily Member 10, Tumor necrosis factor apoptosis inducing ligand splice variant delta, Tumor necrosis factor ligand superfamily member 10

Dichiarazione di non responsabilità

Questo prodotto è solo per uso di ricerca. Non è inteso per uso diagnostico o terapeutico.