The microtiter plate provided in this kit has been pre-coated with an antibody specific to the index. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to the index. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain the index, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of the index in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Piattaforma

Microplate

Tipo di rilevamento

Colorimetric

Tipo di campione

Serum, plasma, tissue homogenates and other biological fluids.

Sensibilità

0.105 ng/ml

Campo di rilevamento

0.312 - 20 ng/ml

Tempo del kit

1 - 4.5 Hours

Reattività

Human

Precision

Intra-assay CV: < 8%, Inter-assay CV: < 10%

Sinonimi

ESCRT I complex subunit TSG101, ESCRT-I complex subunit TSG101, TS101_HUMAN, TSG 10, TSG 101, TSG10, TSG101, Tumor susceptibility 101, Tumor susceptibility gene 10, Tumor susceptibility gene 101 protein, Tumor susceptibility protein, Tumor susceptibility protein isoform 3, VPS 23, VPS23

Dichiarazione di non responsabilità

Questo prodotto è solo per uso di ricerca. Non è inteso per uso diagnostico o terapeutico.