Anti-IKB alpha (phospho Ser32) Anticorpo (A89681)

Name: Anti-IKB alpha (phospho Ser32) Antibody
Brand: Antibodies.com
SKU: A89681

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Anti-IKB alpha (phospho Ser32) Anticorpo (A89681) è stato interrotto e non è più disponibile.

Visualizza tutti gli Anti-IKB alpha Anticorpi.

Descrizione Prodotto

Nome

Anti-IKB alpha (phospho Ser32) Antibody

Descrizione

Rabbit polyclonal antibody to IKB alpha (phospho Ser32).

Applicazioni

WB, IHC, ICC/IF

Diluizioni consigliate

WB: 1:500-1:2,000, IHC: 1:50-1:200, ICC/IF: 1:50-1:200

Reattività

Human, Mouse, Rat

Immunogeno

A synthetic phosphorylated peptide around S32 of human I?Ba (NP_065390.1).

Sequenza

HDSGL

Specie ospite

Rabbit

Clonalità

Polyclonal

Isotipo

IgG

Coniugare

Unconjugated

Purificazione

Affinity purification.

Peso molecolare

39 kDa / 41 kDa

Forma del prodotto

Liquid

Formulazione

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.01% Thiomersal.

Conservazione

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Sinonimi

I kappa B alpha, I-kappa-B-alpha, IkappaBalpha, IkB-alpha, IKBA, IKBalpha, IKBA_HUMAN, MAD 3, MAD3, Major histocompatibility complex enhancer-binding protein MAD3, NF kappa B inhibitor alpha, NF-kappa-B inhibitor alpha, NFKBI, NFKBIA, Nuclear factor of kappa light chain gene enhancer in B cells, Nuclear factor of kappa light polypeptide gene enhancer in B cells inhibitor alpha

Controlli isotipo

Secondari adatti

Dichiarazione di non responsabilità

Questo prodotto è solo per uso di ricerca. Non è inteso per uso diagnostico o terapeutico.

Immagini (8)

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Figure 1: Western Blot - Anti-IKB alpha (phospho Ser32) Antibody (A89681)

Western blot analysis of extracts of HeLa cells, using Anti-IKB alpha (phospho Ser32) Antibody (A89681) at 1:1,000 dilution. HeLa cells were treated by TNF-alpha (20 ng/ml) at 37°C for 30 minutes. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% BSA. Detection was with a ECL Basic Kit. Exposure time: 90s.

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Figure 2: Western Blot - Anti-IKB alpha (phospho Ser32) Antibody (A89681)

Western blot analysis of extracts of HeLa cells, using Anti-IKB alpha (phospho Ser32) Antibody (A89681) at 1:1,000 dilution or IKB alpha antibody (A11397). HeLa cells were treated by TNF-alpha (20 ng/ml) at 37°C for 30 minutes. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% BSA. Detection was with a ECL Basic Kit. Exposure time: 3min.

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Figure 3: Immunohistochemistry - Anti-IKB alpha (phospho Ser32) Antibody (A89681)

Immunohistochemistry analysis of paraffin-embedded human colon tissue using Anti-IKB alpha (phospho Ser32) Antibody (A89681) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

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Figure 4: Immunohistochemistry - Anti-IKB alpha (phospho Ser32) Antibody (A89681)

Immunohistochemistry analysis of paraffin-embedded mouse brain using Anti-IKB alpha (phospho Ser32) Antibody (A89681) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.