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Anti-Ionotropic Glutamate receptor 2 Anticorpo [ARC0572] (A81034)

Nome
Anti-Ionotropic Glutamate receptor 2 Antibody [ARC0572]
Descrizione
Rabbit monoclonal [ARC0572] antibody to Ionotropic Glutamate receptor 2.
Applicazioni
WB, IHC, ICC/IF
Diluizioni consigliate
WB: 1:500-1:2,000, IHC: 1:50-1:200, ICC/IF: 1:50-1:200
Reattività
Human, Mouse, Rat
Immunogeno
A synthetic peptide corresponding to a sequence within amino acids 150-250 of human GluR2/GRIA2 (P42262).
Sequenza
YLYDSDRGLSTLQAVLDSAAEKKWQVTAINVGNINNDKKDEMYRSLFQDLELKKERRVILDCERDKVNDIVDQVITIGKHVKGYHYIIANLGFTDGDLLKI
Specie ospite
Rabbit
Clonalità
Monoclonal
Clona ID
ARC0572
Isotipo
IgG
Coniugare

Unconjugated

Purificazione
Affinity purification.
Peso molecolare
99 kDa
Forma del prodotto
Liquid
Formulazione
Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.02% Sodium Azide.
Conservazione
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Sinonimi
AMPA 2, AMPA selective glutamate receptor 2, AMPA-selective glutamate receptor 2, AMPA2, GluA2, GLUR 2, GLUR B, GluR K2, GluR-2, GluR-B, GluR-K2, GLUR2, GLURB, Glutamate receptor 2, Glutamate receptor ionotropic, Glutamate receptor ionotropic AMPA 2, Gria2, GRIA2_HUMAN, HBGR2
Controlli isotipo
Secondari adatti
Dichiarazione di non responsabilità
Questo prodotto è solo per uso di ricerca. Non è inteso per uso diagnostico o terapeutico.
Western Blot - Anti-Ionotropic Glutamate receptor 2 Antibody [ARC0572] (A81034) - Antibodies.comClicca sull'immagine per la visualizzazione estesa
Figure 1: Western Blot - Anti-Ionotropic Glutamate receptor 2 Antibody [ARC0572] (A81034)
Western blot analysis of extracts of various cell lines, using Anti-Ionotropic Glutamate receptor 2 Antibody [ARC0572] (A81034) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 1s.
Immunohistochemistry - Anti-Ionotropic Glutamate receptor 2 Antibody [ARC0572] (A81034) - Antibodies.comClicca sull'immagine per la visualizzazione estesa
Figure 2: Immunohistochemistry - Anti-Ionotropic Glutamate receptor 2 Antibody [ARC0572] (A81034)
Immunohistochemistry analysis of paraffin-embedded rat brain using Anti-Ionotropic Glutamate receptor 2 Antibody [ARC0572] (A81034) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Immunohistochemistry - Anti-Ionotropic Glutamate receptor 2 Antibody [ARC0572] (A81034) - Antibodies.comClicca sull'immagine per la visualizzazione estesa
Figure 3: Immunohistochemistry - Anti-Ionotropic Glutamate receptor 2 Antibody [ARC0572] (A81034)
Immunohistochemistry analysis of paraffin-embedded mouse brain using Anti-Ionotropic Glutamate receptor 2 Antibody [ARC0572] (A81034) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Immunofluorescence - Anti-Ionotropic Glutamate receptor 2 Antibody [ARC0572] (A81034) - Antibodies.comClicca sull'immagine per la visualizzazione estesa
Figure 4: Immunofluorescence - Anti-Ionotropic Glutamate receptor 2 Antibody [ARC0572] (A81034)
Immunofluorescence analysis of SH-SY5Y cells using Anti-Ionotropic Glutamate receptor 2 Antibody [ARC0572] (A81034) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).

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