Mouse monoclonal [KLC264] antibody to Kappa Light Chain (CF® 488).
Specificità
This antibody is specific to kappa light chain of immunoglobulin and shows no cross-reaction with lambda light chain or any of the five heavy chains. In mammals, the two light chains in an antibody are always identical, with only one type of light chain, kappa or lambda. The ratio of Kappa to Lambda is 70:30. However, with the occurrence of multiple myeloma or other B-cell malignancies this ratio is disturbed. Antibody to the kappa light chain is reportedly useful in the identification of leukemias, plasmacytomas, and certain non-Hodgkins lymphomas. Demonstration of clonality in lymphoid infiltrates indicates that the infiltrate is malignant.
Applicazioni
Flow Cytometry, IF
Diluizioni consigliate
Flow Cytometry: 5 µl / million cells or 5 µl / 100 µl whole blood, IF: 1:50-1:100
Reattività
Human
Immunogeno
Recombinant human Ig kappa chain.
Specie ospite
Mouse
Clonalità
Monoclonal
Clona ID
KLC264
Isotipo
IgG1
Catene leggere
kappa
Coniugare
CF® 488
Excitation: 490nm, Emission: 515nm
Purificazione
Protein A/G chromatography.
Concentrazione
100 µg/ml
Peso molecolare
~22.5 kDa
Forma del prodotto
Liquid
Formulazione
Supplied in 10mM Phosphate Buffered Saline with 0.05% BSA and 0.05% Sodium Azide.