Anti-MCP1 Anticorpo (A12099)

Name: Anti-MCP1 Antibody
Brand: Antibodies.com
SKU: A12099
Price: 335.0 USD
Availability: InStock

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$335

Anticorpo policlonale di coniglio contro MCP1 per WB e IHC.

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Consegna

Spese di spedizione

$40

Tempi di consegna

5-8 Giorni lavorativi

Contattaci

Telefono

+1 (314) 370-6046

E-mail

orders@antibodies.com

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Descrizione Prodotto

Nome

Anti-MCP1 Antibody

Descrizione

Rabbit polyclonal antibody to MCP1.

Applicazioni

WB, IHC

Diluizioni consigliate

WB: 1:1,000-1:5,000, IHC: 1:50-1:200

Reattività

Human, Mouse, Rat

Immunogeno

Recombinant fusion protein containing a sequence corresponding to amino acids 24-99 of human CCL2/MCP-1 (NP_002973.1).

Sequenza

QPDAINAPVTCCYNFTNRKISVQRLASYRRITSSKCPKEAVIFKTIVAKEICADPKQKWVQDSMDHLDKQTQTPKT

Specie ospite

Rabbit

Clonalità

Polyclonal

Isotipo

IgG

Coniugare

Unconjugated

Purificazione

Affinity purification.

Peso molecolare

13 - 15 kDa

Forma del prodotto

Liquid

Formulazione

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.05% Proclin 300.

Conservazione

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Sinonimi

C-C motif chemokine 2, CCL2, CCL2_HUMAN, Chemokine (C C motif) ligand 2, GDCF 2, GDCF-2, GDCF2, HC11, HSMCR30, JE, MCAF, MCP 1, MCP-1, MGC9434, Monocyte chemoattractant protein 1, Monocyte chemotactic and activating factor, Monocyte chemotactic protein 1, Monocyte secretory protein JE, SCYA2, Small inducible cytokine A2, Small inducible cytokine A2 (monocyte chemotactic protein 1, homologous to mouse Sig je), Small inducible cytokine subfamily A (Cys Cys), member 2, Small-inducible cytokine A2, SMC CF, SMC-CF, SMCCF

Controlli isotipo

Secondari adatti

Dichiarazione di non responsabilità

Questo prodotto è solo per uso di ricerca. Non è inteso per uso diagnostico o terapeutico.

Immagini (5)

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Figure 1: Western Blot - Anti-MCP1 Antibody (A12099)

Western blot analysis of A-549, using Anti-MCP1 Antibody (A12099) at 1:2,000 dilution. A-549 cells were treated by TNF-alpha (20 ng/ml) at 37°C for 30 minutes after serum-starvation overnight. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Enhanced Kit (RM00021). Exposure time: 30s.

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Figure 2: Western Blot - Anti-MCP1 Antibody (A12099)

Western blot analysis of various lysates, using Anti-MCP1 Antibody (A12099) at 1:2,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Enhanced Kit (RM00021). Exposure time: 30s.

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Figure 3: Immunohistochemistry - Anti-MCP1 Antibody (A12099)

Immunohistochemistry analysis of paraffin-embedded rat spleen using Anti-MCP1 Antibody (A12099) at a dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Figure 4: Immunohistochemistry - Anti-MCP1 Antibody (A12099)

Immunohistochemistry analysis of paraffin-embedded mouse spleen using Anti-MCP1 Antibody (A12099) at a dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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