Anti-MDC1 Anticorpo [P2B11] (A304945)

Name: Anti-MDC1 Antibody [P2B11]
Brand: Antibodies.com
SKU: A304945
Price: 465.0 USD
Availability: InStock

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$465

Anticorpo monoclonale [P2B11] di topo contro MDC1 per WB e ICC/IF.

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Consegna

Spese di spedizione

$40

Tempi di consegna

5-8 Giorni lavorativi

Contattaci

Telefono

+1 (314) 370-6046

E-mail

orders@antibodies.com

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Descrizione Prodotto

Nome

Anti-MDC1 Antibody [P2B11]

Descrizione

Mouse monoclonal [P2B11] antibody to MDC1.

Specificità

This antibody recognizes MDC1 at and around the N-terminus.

Applicazioni

WB, ICC/IF

Diluizioni consigliate

WB: 1:2,000, ICC/IF: 1:100

Reattività

Human, Mouse, Bovine, Chimpanzee

Immunogeno

GST-tagged recombinant protein corresponding to mouse MDC1 at and around the N-terminus.

Specie ospite

Mouse

Clonalità

Monoclonal

Clona ID

P2B11

Isotipo

IgG1

Coniugare

Unconjugated

Purificazione

Protein G purification.

Concentrazione

1 mg/ml

Peso molecolare

~184 kDa

Forma del prodotto

Liquid

Formulazione

Supplied in Phosphate Buffered Saline, pH 7.4, with 50% Glycerol and 0.09% Sodium Azide.

Conservazione

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Sinonimi

Homologue to Drosophila photoreceptor protein calphotin, MDC 1, MDC1_HUMAN, Mediation of DNA damage checkpoint 1, Mediator of DNA damage checkpoint 1, Mediator of DNA damage checkpoint protein 1, NFBD 1, NFBD1, Nuclear factor with BRCT domains 1, Nuclear Factor with BRCT Domains Protein 1

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Secondari adatti

Dichiarazione di non responsabilità

Questo prodotto è solo per uso di ricerca. Non è inteso per uso diagnostico o terapeutico.

Immagini (3)

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Figure 1: Western Blot - Anti-MDC1 Antibody [P2B11] (A304945)

Western blot analysis of human Embryonic kidney epithelial cell line (HEK293T) lysate showing detection of 184 kDa MDC1 protein using Anti-MDC1 Antibody [P2B11] (A304945) at 1:1,000 for 2 hours room temperature. Lane 1: MW ladder. Lane 2: 293Trap cell lysates. Load: 30 µg. Block: 5% Skim Milk in 1X TBST. The secondary antibody used was Goat Anti-Mouse HRP: IgG at 1:2000 for 60 minutes at room temperature. Color Development: ECL solution for 5 minutes in room temperature. Predicted/Observed Size: 184 kDa.

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Figure 2: Western Blot - Anti-MDC1 Antibody [P2B11] (A304945)

Western blot analysis of mouse Cortex and Cerebellum showing detection of 184 kDa MDC1 protein using Anti-MDC1 Antibody [P2B11] (A304945) at 1:1,000 for 2 hours room temperature. Lane 1: MW ladder. Lane 2: mouse Cortex. Lane 3: mouse Cerebellum. Load: 10 µg. Block: 5% Skim Milk in 1X TBST. The secondary antibody used was Goat Anti-Mouse at 1:2000 for 60 minutes at room temperature. Color Development: ECL solution for 5 minutes in room temperature. Predicted/Observed Size: 184 kDa.

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Figure 3: Immunofluorescence - Anti-MDC1 Antibody [P2B11] (A304945)

Immunocytochemistry/Immunofluorescence analysis of mouse fibroblast cell line (NIH 3T3), fixed in 4% formaldehyde for 15 min at room temperature, using Anti-MDC1 Antibody [P2B11] (A304945), at 1:100 for 60 minutes at room temperature. The secondary antibody used was Goat Anti-Mouse ATTO 488 at 1:100 for 60 minutes at room temperature. Counterstain: DAPI (blue) nuclear stain at 1:5,000 for 5 minutes room temperature. Localization: Nucleus. Magnification: 60X.