Anti-MiTF Anticorpo [ARC55801] (A306180)

Name: Anti-MiTF Antibody [ARC55801]
Brand: Antibodies.com
SKU: A306180

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Questo prodotto è fuori produzione

Anti-MiTF Anticorpo [ARC55801] (A306180) è stato interrotto e non è più disponibile.

Visualizza tutti gli Anti-MiTF Anticorpi.

Descrizione Prodotto

Nome

Anti-MiTF Antibody [ARC55801]

Descrizione

Rabbit monoclonal [ARC55801] antibody to MiTF.

Applicazioni

WB, ICC/IF

Diluizioni consigliate

WB: 1:2,000-1:20,000, ICC/IF: 1:100-1:500

Reattività

Human, Rat

Immunogeno

Recombinant fusion protein containing a sequence corresponding to amino acids 402-526 of human MITF (NP_001341533.1).

Sequenza

HGLSLIPSTGLCSPDLVNRIIKQEPVLENCSQDLLQHHADLTCTTTLDLTDGTITFNNNLGTGTEANQAYSVPTKMGSKLEDILMDDTLSPVGVTDPLLSSVSPGASKTSSRRSSMSMEETEHTC

Specie ospite

Rabbit

Clonalità

Monoclonal

Clona ID

ARC55801

Isotipo

IgG

Coniugare

Unconjugated

Purificazione

Affinity purification.

Peso molecolare

50 - 75 kDa

Forma del prodotto

Liquid

Formulazione

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.05% Proclin 300.

Conservazione

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Sinonimi

bHLHe32, Class E basic helix-loop-helix protein 32, CMM8, Homolog of mouse microphthalmia, Mi, Microphthalmia associated transcription factor, Microphthalmia, mouse, homolog of, Microphthalmia-associated transcription factor, mitfa, MITF_HUMAN, nacre, WS2, WS2A, z3A.1

Controlli isotipo

Secondari adatti

Dichiarazione di non responsabilità

Questo prodotto è solo per uso di ricerca. Non è inteso per uso diagnostico o terapeutico.

Immagini (3)

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Figure 1: Western Blot - Anti-MiTF Antibody [ARC55801] (A306180)

Western blot analysis of extracts of Hela cells, using Anti-MiTF Antibody [ARC55801] (A306180) at 1:20,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 30s.

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Figure 2: Western Blot - Anti-MiTF Antibody [ARC55801] (A306180)

Western blot analysis of extracts of Rat heart, using Anti-MiTF Antibody [ARC55801] (A306180) at 1:20,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Enhanced Kit (RM00021). Exposure time: 180s.

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Figure 3: Immunofluorescence - Anti-MiTF Antibody [ARC55801] (A306180)

Immunofluorescence analysis of HeLa using Anti-MiTF Antibody [ARC55801] (A306180) at a dilution of 1:300 (40x lens). DAPI was used to stain the cell nuclei (blue).