Topo IgG1 - Isotype Control (A284021)

Mouse IgG1 - Isotype Control

Flow Cytometry

Use 10µl of the suggested working dilution to label 10⁶ cells or 100µl whole blood

Mouse

Monoclonal

IgG1

Unconjugated

Protein A affinity chromatography of tissue culture supernatant.

100 µg/ml

Supplied in Phosphate Buffered Saline with 1% BSA and 0.09% Sodium Azide.

Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended.

Mouse IgG1 negative control is negative by flow cytometry on all human cells and cell lines tested. Further tests have also shown that this reagent is also suitable for use as a negative control with bovine (Maslanka et al, 2012), ovine, porcine (Kapetanovic et al, 2012), equine (Jacks et al, 2007), canine (Maiolini et al, 2012) , lapine (Pakandl et al, 2008) and guinea-pig tissues.This reagent recognizes a rat cell surface marker, and therefore cannot be used as a negative control in this species.