Mouse IL-12 p70 ELISA Kit is based on the quantitative sandwich enzyme-linked immunosorbent assay technique to measure concentration of mouse IL-12 p70 in the samples. A monoclonal antibody specific for mouse IL-12 p70 has been immobilized onto microwells. Standard or samples are pipetted into the wells, followed by the addition of biotin-linked monoclonal antibody specific for IL-12 p70, and IL-12 p70 present is bound by the immobilized antibody and detect antibody following the first incubation. After removal of any unbound substances, streptavidin-HRP is added for a second incubation. After washing, substrate solution reacts with HRP and color develops in proportion to the amount of IL-12 p70 bound by the immobilized antibody. The color development is stopped by addition of acid and the optical density value is measured by microplate reader.
Piattaforma
Microplate
Tipo di rilevamento
Colorimetric
Tipo di campione
Cell culture supernatant, serum and plasma.
Sensibilità
3.58 pg/ml
Campo di rilevamento
31.25 - 2000 pg/ml
Recupero
99% (Average Recovery from Serum Samples).
Specificità
This kit can assay both natural and recombinant mouse IL-12 p70.
Reattività
Mouse
Reattività incrociata
This assay has shown no significant cross-reactivity.
Precision
Intra-assay CV: < 8%, Inter-assay CV: < 10%
Conservazione
Store at 2 - 8°C.
Sinonimi
CLMF p35, CLMF p40, Cytotoxic lymphocyte maturation factor 35 kDa subunit , Cytotoxic lymphocyte maturation factor 40 kDa subunit, IL 12A, IL 12B, NK cell stimulatory factor chain 1, NK cell stimulatory factor chain 2
Dichiarazione di non responsabilità
Questo prodotto è solo per uso di ricerca. Non è inteso per uso diagnostico o terapeutico.
