The microtiter plate provided in this kit has been pre-coated with an antibody specific to the index. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to the index. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain the index, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of the index in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Piattaforma

Microplate

Tipo di rilevamento

Colorimetric

Tipo di campione

Serum, plasma, tissue homogenates and other biological fluids.

Sensibilità

0.112 ng/ml

Campo di rilevamento

0.312 - 20 ng/ml

Tempo del kit

1 - 4.5 Hours

Reattività

Mouse

Precision

Intra-assay CV: < 8%, Inter-assay CV: < 10%

Sinonimi

Calcium binding protein intestinal related, Endoplasmic reticulum resident protein 70, Endoplasmic reticulum resident protein 72, EPR70, EPR72, ER protein 70, ER protein 72, ERP 70, ERp 72, ERp-72, ERp70, ERp72, PDIA 4, Pdia4, PDIA4_HUMAN, PDIR, Protein disulfide isomerase associated 4, Protein disulfide isomerase family A member 4, Protein disulfide isomerase related protein, protein disulfide isomerase related protein (calcium-binding protein, intestinal-related), Protein disulfide-isomerase A4, Protein ERp 72, Protein ERp72

Dichiarazione di non responsabilità

Questo prodotto è solo per uso di ricerca. Non è inteso per uso diagnostico o terapeutico.