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Anti-MSH6 Anticorpo [ARC57727] (A309410)

Nome
Anti-MSH6 Antibody [ARC57727]
Descrizione
Rabbit monoclonal [ARC57727] antibody to MSH6.
Applicazioni
WB, IHC, ICC/IF
Diluizioni consigliate
WB: 1:1,000-1:5,000, IHC: 1:100-1:500, ICC/IF: 1:50-1:200
Reattività
Human, Mouse, Rat
Immunogeno
A synthesized peptide derived from human MSH6.
Specie ospite
Rabbit
Clonalità
Monoclonal
Clona ID
ARC57727
Isotipo
IgG
Coniugare

Unconjugated

Purificazione
Affinity purification.
Forma del prodotto
Liquid
Formulazione
Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.05% Proclin 300.
Conservazione
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Sinonimi
DNA mismatch repair protein Msh6, G/T mismatch binding protein, G/T mismatch-binding protein, GTBP, GTMBP, hMSH6, HNPCC 5, HNPCC5, HSAP, MSH 6, MSH6_HUMAN, mutS (E. coli) homolog 6, MutS alpha 160 kDa subunit, mutS homolog 6, MutS homolog 6 (E. coli), MutS-alpha 160 kDa subunit, p160, Sperm associated protein
Controlli isotipo
Secondari adatti
Dichiarazione di non responsabilità
Questo prodotto è solo per uso di ricerca. Non è inteso per uso diagnostico o terapeutico.
Western Blot - Anti-MSH6 Antibody [ARC57727] (A309410) - Antibodies.comClicca sull'immagine per la visualizzazione estesa
Figure 1: Western Blot - Anti-MSH6 Antibody [ARC57727] (A309410)
Western blot analysis of various lysates, using Anti-MSH6 Antibody [ARC57727] (A309410) at 1:3,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 3s.
Western Blot - Anti-MSH6 Antibody [ARC57727] (A309410) - Antibodies.comClicca sull'immagine per la visualizzazione estesa
Figure 2: Western Blot - Anti-MSH6 Antibody [ARC57727] (A309410)
Western blot analysis of Rat testis, using Anti-MSH6 Antibody [ARC57727] (A309410) at 1:3,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 3s.
Western Blot - Anti-MSH6 Antibody [ARC57727] (A309410) - Antibodies.comClicca sull'immagine per la visualizzazione estesa
Figure 3: Western Blot - Anti-MSH6 Antibody [ARC57727] (A309410)
Western blot analysis of extracts from wild type(WT) and MSH6 knockout (KO) 293T cells, using Anti-MSH6 Antibody [ARC57727] (A309410) at 1:3,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 3s.
Immunohistochemistry - Anti-MSH6 Antibody [ARC57727] (A309410) - Antibodies.comClicca sull'immagine per la visualizzazione estesa
Figure 4: Immunohistochemistry - Anti-MSH6 Antibody [ARC57727] (A309410)
Immunohistochemistry analysis of paraffin-embedded human appendix tissue using Anti-MSH6 Antibody [ARC57727] (A309410) at a dilution of 1:400 (40x lens). Perform high pressure antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

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