This antibody recognises chondroitin sulfate proteoglycan NG2 also known as chondroitin sulfate proteoglycan 4 (CSPG4) and melanoma chondroitin sulfate proteoglycan. It was first characterized in rat as a high-molecular-weight type 1 membrane proteoglycan (Stallcup 1981). CSPG4 is involved in cell attachment and migration. It regulates spreading of melanoma cells by recruiting and activating a signalling cascade through CDC42, ACK1 and p130cas. It enhances FAK and ERK activation.

Applicazioni

Diluizioni consigliate

WB: 1:500 - 1:2,000

Reattività

Human

Immunogeno

Recombinant fusion protein containing a sequence corresponding to amino acids 1950-2210 of human chondroitin sulphate proteoglycan NG2.

Sequenza

LEVPQALGRSSLSQQQLRVVSDREEPEAAYRLIQGPQYGHLLVGGRPTSAFSQFQIDQGEVVFAFTNFSSSHDHFRVLALARGVNASAVVNVTVRALLHVWAGGPWPQGATLRLDPTVLDAGELANRTGSVPRFRLLEGPRHGRVVRVPRARTEPGGSQLVEQFTQQDLEDGRLGLEVGRPEGRAPGPAGDSLTLELWAQGVPPAVASLDFATEPYNAARPYSVALLSVPEAARTEAGKPESSTPTGEPGPMASSPEPAVA

Specie ospite

Rabbit

Clonalità

Polyclonal

Isotipo

IgG

Coniugare

Unconjugated

Concentrazione

Lot Specific

Peso molecolare

Approximately 320 kDa in HeLa and A375 cell lysates under reducing conditions

Forma del prodotto

Liquid

Formulazione

Supplied in Phosphate Buffered Saline with 0.05% ProClin 300 and 50% Glycerol.

Conservazione

Store undiluted at -20°C. Storage in frost free freezers is not recommended. Avoid freeze-thaw cycles. Should this product contain a precipitate we recommend microcentrifugation before use.

Note generali

Rabbit anti chondroitin sulphate proteoglycan NG2 antibody recognizes chondroitin sulfate proteoglycan NG2 also known as chondroitin sulfate proteoglycan 4 (CSPG4) and melanoma chondroitin sulfate proteoglycan. It was first characterized in rat as a high-molecular-weight type 1 membrane proteoglycan (Stallcup 1981). CSPG4 is involved in cell attachment and migration (Karram et al. 2005). It regulates spreading of melanoma cells by recruiting and activating a signalling cascade through CDC42, ACK1 and p130cas (Eisenmann et al. 1999). It enhances FAK and ERK activation (Yang et al. 2004).

Sinonimi

4732461B14Rik, AN2, AN2 proteoglycan, Chondroitin sulfate proteoglycan 4, Chondroitin sulfate proteoglycan 4 (melanoma-associated), Chondroitin sulfate proteoglycan NG2, Cspg4, Cspg4 chondroitin sulfate proteoglycan 4, CSPG4_HUMAN, HMW-MAA, HSN tumor-specific antigen, Kiaa4232, MCSP, MCSPG, MEL-CSPG, Melanoma chondroitin sulfate proteoglycan, Melanoma-associated chondroitin sulfate proteoglycan, MELCSPG, MSK16

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Secondari adatti

Dichiarazione di non responsabilità

Questo prodotto è solo per uso di ricerca. Non è inteso per uso diagnostico o terapeutico.