Anti-NMDAR2A Anticorpo [S327A-38] (A304975)

Name: Anti-NMDAR2A Antibody [S327A-38]
Brand: Antibodies.com
SKU: A304975
Price: 500.0 USD
Availability: InStock

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$500

Anticorpo monoclonale [S327A-38] di topo contro NMDAR2A per WB, IHC e ICC/IF.

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Consegna

Spese di spedizione

$40

Tempi di consegna

5-8 Giorni lavorativi

Contattaci

Telefono

+1 (314) 370-6046

E-mail

orders@antibodies.com

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Descrizione Prodotto

Nome

Anti-NMDAR2A Antibody [S327A-38]

Descrizione

Mouse monoclonal [S327A-38] antibody to NMDAR2A.

Specificità

This antibody does not react with NR2B.

Applicazioni

WB, IHC, ICC/IF

Diluizioni consigliate

WB: 1:1,000, ICC/IF: 1:100

Reattività

Human, Mouse, Rat

Immunogeno

Fusion protein amino acids 75-325 (extracellular N-terminus) of rat GluN2A/NR2A.

Specie ospite

Mouse

Clonalità

Monoclonal

Clona ID

S327A-38

Isotipo

IgG2b

Coniugare

Unconjugated

Purificazione

Protein G purification.

Concentrazione

1 mg/ml

Peso molecolare

~170 kDa

Forma del prodotto

Liquid

Formulazione

Supplied in Phosphate Buffered Saline, pH 7.4, with 50% Glycerol and 0.09% Sodium Azide.

Conservazione

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Sinonimi

EPND, FESD, GluN2A, Glutamate receptor, Glutamate receptor ionotropic N methyl D aspartate 2A, Glutamate [NMDA] receptor subunit epsilon-1, GRIN 2A, GRIN2A, hNR2A, LKS, N methyl D aspartate receptor channel, subunit epsilon 1, N Methyl D Aspartate Receptor Subtype 2A, N methyl D aspartate receptor subunit 2A, N-methyl D-aspartate receptor subtype 2A, NMDA receptor subtype 2A, NMDAR 2A, NMDE1_HUMAN, NR2A, OTTHUMP00000160135, OTTHUMP00000174531

Controlli isotipo

Secondari adatti

Dichiarazione di non responsabilità

Questo prodotto è solo per uso di ricerca. Non è inteso per uso diagnostico o terapeutico.

Immagini (3)

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Figure 1: Immunofluorescence - Anti-NMDAR2A Antibody [S327A-38] (A304975)

Immunocytochemistry/Immunofluorescence analysis of human neuroblastoma cell line (SK-N-BE, fixed in 4% formaldehyde for 15 min at room temperature, using Anti-NMDAR2A Antibody [S327A-38] (A304975), at 1:100 for 60 minutes at room temperature. The secondary antibody used was Goat Anti-Mouse ATTO 488 at 1:200 for 60 minutes at room temperature. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5,000 for 60 minutes at room temperature, 5 minutes at room temperature. Localization: Cell Membrane, Cytoplasm. Magnification: 60X.(A) Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain. (B) Anti-GluN2A/NR2A Antibody. (C) Composite.(A) DAPI (blue) nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) GluN2A/NR2A Antibody. (D) Composite.

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Figure 2: Western Blot - Anti-NMDAR2A Antibody [S327A-38] (A304975)

Western blot analysis of Monkey COS cells transfected with GFP-tagged NR2A showing detection of ~170 kDa GluN2A/NR2A protein using Anti-NMDAR2A Antibody [S327A-38] (A304975) at 1:200 for 16 hours at 4°C. Lane 1: Molecular Weight Ladder. Lane 2: Monkey COS cells transfected with GFP-tagged NR2A. Load: 15 µg. Block: 2% BSA and 2% Skim Milk in 1X TBST. The secondary antibody used was Goat Anti-Mouse IgG: HRP at 1:1,000 for 1 hour room temperature. Color Development: ECL solution for 6 min in room temperature. Predicted/Observed Size: ~170 kDa.

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Figure 3: Immunofluorescence - Anti-NMDAR2A Antibody [S327A-38] (A304975)

Immunocytochemistry/Immunofluorescence analysis of human neuroblastoma cells (SH-SY5Y), fixed in 4% PFA for 15 min, using Anti-NMDAR2A Antibody [S327A-38] (A304975), at 1:100 for overnight at 4°C with slow rocking. The secondary antibody used was AlexaFluor 488 at 1:1,000 for 1 hour at room temperature. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 minutes at room temperature.(A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) GluN2A/NR2A Antibody (D) Composite.