Anti-Nrf2 Anticorpo (A80763)

Name: Anti-Nrf2 Antibody
Brand: Antibodies.com
SKU: A80763
Price: 335.0 USD
Availability: InStock

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$335

Anticorpo policlonale di coniglio contro Nrf2 per WB e IHC.

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Consegna

Spese di spedizione

$40

Tempi di consegna

5-8 Giorni lavorativi

Contattaci

Telefono

+1 (314) 370-6046

E-mail

orders@antibodies.com

Garanzie

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Descrizione Prodotto

Nome

Anti-Nrf2 Antibody

Descrizione

Rabbit polyclonal antibody to Nrf2.

Applicazioni

WB, IHC

Diluizioni consigliate

WB: 1:500-1:1,000, IHC: 1:50-1:200

Reattività

Human, Mouse, Rat

Immunogeno

Recombinant fusion protein containing a sequence corresponding to amino acids 243-400 of human NRF2 (NP_006155.2).

Sequenza

SPHFLNAFEDSFSSILSTEDPNQLTVNSLNSDATVNTDFGDEFYSAFIAEPSISNSMPSPATLSHSLSELLNGPIDVSDLSLCKAFNQNHPESTAEFNDSDSGISLNTSPSVASPEHSVESSSYGDTLLGLSDSEVEELDSAPGSVKQNGPKTPVHSS

Specie ospite

Rabbit

Clonalità

Polyclonal

Isotipo

IgG

Coniugare

Unconjugated

Purificazione

Affinity purification.

Peso molecolare

97 - 110 kDa

Forma del prodotto

Liquid

Formulazione

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.05% Proclin 300.

Conservazione

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Sinonimi

erythroid derived 2, HEBP1, like 2, NF E2 related factor 2, NF-E2-related factor 2, NF2L2_HUMAN, NFE2 related factor 2, NFE2-related factor 2, Nfe2l2, Nrf 2, Nuclear factor, Nuclear factor (erythroid derived 2) like 2, nuclear factor erythroid 2 like 2, Nuclear factor erythroid 2 related factor 2, Nuclear factor erythroid 2-related factor 2, Nuclear factor erythroid derived 2 like 2

Controlli isotipo

Secondari adatti

Dichiarazione di non responsabilità

Questo prodotto è solo per uso di ricerca. Non è inteso per uso diagnostico o terapeutico.

Immagini (5)

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Figure 1: Western Blot - Anti-Nrf2 Antibody (A80763)

Western blot analysis of various lysates, using Anti-Nrf2 Antibody (A80763) at 1:1,000 dilution. HeLa cells were treated by MG132(50 µM) at 37°C for 90 minutes. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 30s.

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Figure 2: Western Blot - Anti-Nrf2 Antibody (A80763)

Western blot analysis of various lysates, using Anti-Nrf2 Antibody (A80763) at 1:1,000 dilution. NIH3T3 cells were treated by MG132(50 µM) at 37°C for 90 minutes. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 30s.

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Figure 3: Western Blot - Anti-Nrf2 Antibody (A80763)

Western blot analysis of various lysates, using Anti-Nrf2 Antibody (A80763) at 1:1,000 dilution. C6 cells were treated by MG132(50 µM) at 37°C for 90 minutes. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 30s.

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Figure 4: Immunohistochemistry - Anti-Nrf2 Antibody (A80763)

Immunohistochemistry analysis of paraffin-embedded human breast cancer tissue using Anti-Nrf2 Antibody (A80763) at a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.