Anti-PCK2 Anticorpo [ARC1017] (A305761)

Name: Anti-PCK2 Antibody [ARC1017]
Brand: Antibodies.com
SKU: A305761

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Questo prodotto è fuori produzione

Anti-PCK2 Anticorpo [ARC1017] (A305761) è stato interrotto e non è più disponibile.

Visualizza tutti gli Anti-PCK2 Anticorpi.

Descrizione Prodotto

Nome

Anti-PCK2 Antibody [ARC1017]

Descrizione

Rabbit monoclonal [ARC1017] antibody to PCK2.

Applicazioni

WB, ICC/IF

Diluizioni consigliate

WB: 1:500-1:1,000, ICC/IF: 1:50-1:200

Reattività

Human, Mouse, Rat

Immunogeno

Recombinant fusion protein containing a sequence corresponding to amino acids 550-640 of human PEPCK/PEPCK/PCK2 (Q16822).

Sequenza

ENARVLDWICRRLEGEDSARETPIGLVPKEGALDLSGLRAIDTTQLFSLPKDFWEQEVRDIRSYLTEQVNQDLPKEVLAELEALERRVHKM

Specie ospite

Rabbit

Clonalità

Monoclonal

Clona ID

ARC1017

Isotipo

IgG

Coniugare

Unconjugated

Purificazione

Affinity purification.

Peso molecolare

71 kDa

Forma del prodotto

Liquid

Formulazione

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.02% Sodium Azide.

Conservazione

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Sinonimi

EC 4.1.1.32, GTP mitochondrial precursor, HGNC:8725, mitochondrial, Mitochondrial phosphoenolpyruvate carboxykinase 2, OTTHUMP00000164700, PCKGM_HUMAN, PE, PEP carboxykinase, PEPCK, PEPCK deficiency mitochondrial, PEPCK M, PEPCK-M, PEPCK2, Phosphoenolpyruvate carboxykinase 2 (mitochondrial), Phosphoenolpyruvate carboxykinase 2 mitochondrial, Phosphoenolpyruvate carboxykinase [GTP], Phosphoenolpyruvate carboxylase, Phosphopyruvate carboxylase

Controlli isotipo

Secondari adatti

Dichiarazione di non responsabilità

Questo prodotto è solo per uso di ricerca. Non è inteso per uso diagnostico o terapeutico.

Immagini (3)

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Figure 1: Western Blot - Anti-PCK2 Antibody [ARC1017] (A305761)

Western blot analysis of extracts of various cell lines, using Anti-PCK2 Antibody [ARC1017] (A305761) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 1s.

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Figure 2: Western Blot - Anti-PCK2 Antibody [ARC1017] (A305761)

Western blot analysis of extracts from wild type(WT) and PEPCK/PCK2 knockout (KO) 293T cells, using Anti-PCK2 Antibody [ARC1017] (A305761) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 30s.

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Figure 3: Immunofluorescence - Anti-PCK2 Antibody [ARC1017] (A305761)

Immunofluorescence analysis of HeLa cells using Anti-PCK2 Antibody [ARC1017] (A305761) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).