Anti-PHD3 Anticorpo [ARC52452] (A307618)

Name: Anti-PHD3 Antibody [ARC52452]
Brand: Antibodies.com
SKU: A307618

Clicca sull'immagine per la visualizzazione estesa

Questo prodotto è fuori produzione

Anti-PHD3 Anticorpo [ARC52452] (A307618) è stato interrotto e non è più disponibile.

Visualizza tutti gli Anti-PHD3 Anticorpi.

Descrizione Prodotto

Nome

Anti-PHD3 Antibody [ARC52452]

Descrizione

Rabbit monoclonal [ARC52452] antibody to PHD3.

Applicazioni

WB, IHC, ICC/IF

Diluizioni consigliate

WB: 1:2,000-1:20,000, IHC: 1:500-1:1,000, ICC/IF: 1:50-1:200

Reattività

Human, Mouse, Rat

Immunogeno

A synthetic peptide corresponding to a sequence within amino acids 1-100 of human PHD3 (NP_071356.1).

Sequenza

MPLGHIMRLDLEKIALEYIVPCLHEVGFCYLDNFLGEVVGDCVLERVKQLHCTGALRDGQLAGPRAGVSKRHLRGDQITWIGGNEEGCEAISFLLSLIDR

Specie ospite

Rabbit

Clonalità

Monoclonal

Clona ID

ARC52452

Isotipo

IgG

Coniugare

Unconjugated

Purificazione

Affinity purification.

Peso molecolare

32 kDa

Forma del prodotto

Liquid

Formulazione

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.05% Proclin 300.

Conservazione

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Sinonimi

Egl 9 family hypoxia inducible factor 3, Egl nine homolog 3, Egl nine homolog 3 (C. elegans), Egl nine like protein 3 isoform, EGL9 homolog of C. elegans 3, EGLN3, EGLN3_HUMAN, Factor responsive smooth muscle protein, HIF Prolyl Hydroxylase 3, HIF-PH3, HIF-prolyl hydroxylase 3, HIFP4H3, HIFPH3, HPH-1, HPH-3, Hypoxia-inducible factor prolyl hydroxylase 3, P4H3, Prolyl Hydroxylase Domain Containing Protein 3, Prolyl hydroxylase domain-containing protein 3, SM20

Controlli isotipo

Secondari adatti

Dichiarazione di non responsabilità

Questo prodotto è solo per uso di ricerca. Non è inteso per uso diagnostico o terapeutico.

Immagini (7)

Clicca sull'immagine per la visualizzazione estesa

Figure 1: Western Blot - Anti-PHD3 Antibody [ARC52452] (A307618)

Western blot analysis of extracts of various cell lines, using Anti-PHD3 Antibody [ARC52452] (A307618) at 1:20,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 60s.

Clicca sull'immagine per la visualizzazione estesa

Figure 2: Western Blot - Anti-PHD3 Antibody [ARC52452] (A307618)

Western blot analysis of extracts of BxPC-3 cells, using Anti-PHD3 Antibody [ARC52452] (A307618) at 1:20,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 90s.

Clicca sull'immagine per la visualizzazione estesa

Figure 3: Immunohistochemistry - Anti-PHD3 Antibody [ARC52452] (A307618)

Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue using Anti-PHD3 Antibody [ARC52452] (A307618) at a dilution of 1:1,000 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Clicca sull'immagine per la visualizzazione estesa

Figure 4: Immunohistochemistry - Anti-PHD3 Antibody [ARC52452] (A307618)

Immunohistochemistry analysis of paraffin-embedded human esophageal cancer using Anti-PHD3 Antibody [ARC52452] (A307618) at a dilution of 1:1,000 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.