Anti-PRPF4 Anticorpo [ARC3034] (A309448)

Western blot analysis of extracts of various cell lines, using Anti-PRPF4 Antibody [ARC3034] (A309448) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 30s.

Western blot analysis of extracts of Rat liver, using Anti-PRPF4 Antibody [ARC3034] (A309448) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 180s.

Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue using Anti-PRPF4 Antibody [ARC3034] (A309448) at a dilution of 1:50(40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Immunohistochemistry analysis of paraffin-embedded human esophagus using Anti-PRPF4 Antibody [ARC3034] (A309448) at a dilution of 1:50(40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.