Anti-PTEN Anticorpo [ARC2643] (A307897)

Name: Anti-PTEN Antibody [ARC2643]
Brand: Antibodies.com
SKU: A307897

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Questo prodotto è fuori produzione

Anti-PTEN Anticorpo [ARC2643] (A307897) è stato interrotto e non è più disponibile.

Visualizza tutti gli Anti-PTEN Anticorpi.

Descrizione Prodotto

Nome

Anti-PTEN Antibody [ARC2643]

Descrizione

Rabbit monoclonal [ARC2643] antibody to PTEN.

Applicazioni

WB, IHC, ICC/IF, IP

Diluizioni consigliate

WB: 1:500-1:1,000, IHC: 1:50-1:200, ICC/IF: 1:50-1:200, IP: 1:500-1:1,000

Reattività

Human, Mouse, Rat

Immunogeno

Recombinant fusion protein containing a sequence corresponding to amino acids 1-403 of human PTEN (P60484).

Sequenza

MTAIIKEIVSRNKRRYQEDGFDLDLTYIYPNIIAMGFPAERLEGVYRNNIDDVVRFLDSKHKNHYKIYNLCAERHYDTAKFNCRVAQYPFEDHNPPQLELIKPFCEDLDQWLSEDDNHVAAIHCKAGKGRTGVMICAYLLHRGKFLKAQEALDFYGEVRTRDKKGVTIPSQRRYVYYYSYLLKNHLDYRPVALLFHKMMFETIPMFSGGTCNPQFVVCQLKVKIYSSNSGPTRREDKFMYFEFPQPLPVCGDIKVEFFHKQNKMLKKDKMFHFWVNTFFIPGPEETSEKVENGSLCDQEIDSICSIERADNDKEYLVLTLTKNDLDKANKDKANRYFSPNFKVKLYFTKTVEEPSNPEASSSTSVTPDVSDNEPDHYRYSDTTDSDPENEPFDEDQHTQITKV

Specie ospite

Rabbit

Clonalità

Monoclonal

Clona ID

ARC2643

Isotipo

IgG

Coniugare

Unconjugated

Purificazione

Affinity purification.

Peso molecolare

54 kDa

Forma del prodotto

Liquid

Formulazione

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.02% Sodium Azide.

Conservazione

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Sinonimi

10q23del, BZS, DEC, GLM2, MGC11227, MHAM, MMAC1, MMAC1 phosphatase and tensin homolog deleted on chromosome 10, Mutated in multiple advanced cancers 1, Phosphatase and tensin homolog, Phosphatase and tensin like protein, Phosphatidylinositol 3,4,5-trisphosphate 3-phosphatase and dual-specificity protein phosphatase PTEN, PTEN1, PTEN_HUMAN, TEP1

Controlli isotipo

Secondari adatti

Dichiarazione di non responsabilità

Questo prodotto è solo per uso di ricerca. Non è inteso per uso diagnostico o terapeutico.

Immagini (6)

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Figure 1: Western Blot - Anti-PTEN Antibody [ARC2643] (A307897)

Western blot analysis of extracts of various cell lines, using Anti-PTEN Antibody [ARC2643] (A307897) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 1s.

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Figure 2: Western Blot - Anti-PTEN Antibody [ARC2643] (A307897)

Western blot analysis of extracts of C6 cells, using Anti-PTEN Antibody [ARC2643] (A307897) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 60s.

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Figure 3: Western Blot - Anti-PTEN Antibody [ARC2643] (A307897)

Western blot analysis of extracts from wild type (WT) and PTEN knockout (KO) HeLa cells, using Anti-PTEN Antibody [ARC2643] (A307897) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 60s.

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Figure 4: Immunohistochemistry - Anti-PTEN Antibody [ARC2643] (A307897)

Immunohistochemistry analysis of paraffin-embedded human breast cancer tissue using Anti-PTEN Antibody [ARC2643] (A307897) at a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.