Rat IL-6 ELISA Kit is based on the quantitative sandwich enzyme-linked immunosorbent assay technique to measure concentration of rat IL-6 in the samples. A monoclonal antibody specific for rat IL-6 has been immobilized onto microwells. Standard or samples are pipetted into the wells, followed by the addition of biotin-linked monoclonal antibody specific for IL-6, and IL-6 present is bound by the immobilized antibody and detect antibody following the first incubation. After removal of any unbound substances, streptavidin-HRP is added for a second incubation. After washing, substrate solution reacts with HRP and color develops in proportion to the amount of IL-6 bound by the immobilized antibody. The color development is stopped by addition of acid and the optical density value is measured by microplate reader.

Piattaforma

Microplate

Tipo di rilevamento

Colorimetric

Tipo di campione

Cell culture supernatant, serum and plasma.

Sensibilità

2.57 pg/ml

Campo di rilevamento

31.25 - 2000 pg/ml

Recupero

97% (Average Recovery from Serum Samples).

Specificità

This kit can assay both natural and recombinant rat IL-6.

Reattività

Rat

Reattività incrociata

This assay has shown no significant cross-reactivity.

Precision

Intra-assay CV: < 8%, Inter-assay CV: < 10%

Conservazione

Store at 2 - 8°C.

Sinonimi

B cell differentiation factor , B cell stimulatory factor 2, B-cell stimulatory factor 2, BSF 2, BSF-2, BSF2, CDF, CTL differentiation factor, Cytotoxic T cell differentiation factor , Hepatocyte stimulating fact, Hepatocyte stimulating factor, Hepatocyte stimulatory factor, HGF, HPGF, HSF , Hybridoma growth factor, Hybridoma growth factor Interferon beta-2, Hybridoma plasmacytoma growth factor , IFN-beta-2, IFNB2 , IL 6, IL6, IL6 protein , IL6_HUMAN, Interferon beta 2, Interferon beta-2, Interleukin 6, Interleukin 6 (interferon beta 2) , Interleukin BSF 2, Interleukin BSF2, Interleukin-6, Interleukin6

Dichiarazione di non responsabilità

Questo prodotto è solo per uso di ricerca. Non è inteso per uso diagnostico o terapeutico.