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Ricombinante Bet v 1 (1.0101) proteinaa (A242914)

Flow Cytometry - Recombinant Bet v 1 (1.0101) Protein (A242913) - Antibodies.com SDS-PAGE - Recombinant Bet v 1 (1.0101) Protein (A242914) - Antibodies.com ELISA - Recombinant Bet v 1 (1.0101) Protein (A242914) - Antibodies.com
$525
Product Datasheet
Garanzia al 100%
Garanzia di corrispondenza del prezzo

Spese di spedizione
$40
Tempi di consegna
4-6 Giorni lavorativi

Telefono
+1 (314) 370-6046
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orders@antibodies.com

Nome
Recombinant Bet v 1 (1.0101) Protein
Applicazioni
Flow Cytometry, ELISA
Sistema di espressione
Escherichia coli
Tipo di proteina
Recombinant
Specie proteiche
Betula verrucosa (Silver Birch)
Sequenza
DNA sequence encoding 172 amino acids was fused with a Strep Tag at the N-terminus to form this recombinant protein.
Etichetta
Strep Tag (N-terminus)
MW previsto
19 kDa
Coniugare

Unconjugated

Purificazione
Ion exchange and affinity chromatography.
Forma del prodotto
Lyophilized
Concentrazione
Reconstitution dependent.
Formulazione
Lyophilized from 100mM Tris Buffer, pH 8, with 150mM NaCl, and without preservatives or carriers (0.2 µm filter sterilized).
Conservazione
Shipped at 4°C. Lyophilized: Store at -20°C to -80°C. Reconstituted: Use immediately or aliquot and store at -20°C to -80°C. Avoid freeze/thaw cycles.
Dichiarazione di non responsabilità
Questo prodotto è solo per uso di ricerca. Non è inteso per uso diagnostico o terapeutico.
Flow Cytometry - Recombinant Bet v 1 (1.0101) Protein (A242913) - Antibodies.comClicca sull'immagine per la visualizzazione estesa
Figure 1: Flow Cytometry - Recombinant Bet v 1 (1.0101) Protein (A242914)
Flow cytometry dot-plot staining pattern of Recombinant Bet v 1 (1.0101) Protein stimulated human peripheral whole blood lymphocytes and basophils of a proven allergic donor stained using Anti-CD63 [MEM-259] (FITC) Antibody and Anti-CD203c [NP4D6] (PE) Antibody.
SDS-PAGE - Recombinant Bet v 1 (1.0101) Protein (A242914) - Antibodies.comClicca sull'immagine per la visualizzazione estesa
Figure 2: SDS-PAGE - Recombinant Bet v 1 (1.0101) Protein (A242914)
Purity verification: 5 µg of Recombinant Bet v 1 (1.0101) Protein with > 95 % purity checked by Coomassie Brilliant Blue stained SDS-PAGE.
ELISA - Recombinant Bet v 1 (1.0101) Protein (A242914) - Antibodies.comClicca sull'immagine per la visualizzazione estesa
Figure 3: Recombinant Bet v 1 (1.0101) Protein (A242914)
An ELISA test was designed to prove the bond between the coated target, Recombinant Bet v 1 (1.0101) Protein, and allergen-specific human plasma IgG4 antibodies of Betula verrucosa positive donor. A measurable signal was subsequently generated by the addition of Anti-Human IgG4 Antibody (Biotin), Streptavidin-HRP, and substrate solution (TMB). The intensity of the signal is proportional to the amount of coated Recombinant Bet v 1 (1.0101) Protein.
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