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Ricombinante Phl p 7 (7.0101) proteinaa (A242916)

Flow Cytometry - Recombinant Phl p 7 (7.0101) Protein (A242915) - Antibodies.com SDS-PAGE - Recombinant Phl p 7 (7.0101) Protein (A242916) - Antibodies.com ELISA - Recombinant Phl p 7 (7.0101) Protein (A242916) - Antibodies.com
$525
Product Datasheet
Garanzia al 100%
Garanzia di corrispondenza del prezzo

Spese di spedizione
$40
Tempi di consegna
4-6 Giorni lavorativi

Telefono
+1 (314) 370-6046
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orders@antibodies.com

Nome
Recombinant Phl p 7 (7.0101) Protein
Applicazioni
ELISA, Flow Cytometry, WB
Sistema di espressione
Escherichia coli
Tipo di proteina
Recombinant
Specie proteiche
Phleum pratense (Timothy Grass)
Sequenza
DNA sequence encoding 90 amino acids was fused with a Strep Tag at the N-terminus to form this recombinant protein.
Etichetta
Strep Tag (N-terminus)
MW previsto
10 kDa
Coniugare

Unconjugated

Purificazione
Ion exchange and affinity chromatography.
Forma del prodotto
Lyophilized
Concentrazione
Reconstitution dependent.
Formulazione
Lyophilized from 100mM Tris Buffer, pH 8, with 150mM NaCl, and without preservatives or carriers (0.2 µm filter sterilized).
Conservazione
Shipped at 4°C. Lyophilized: Store at -20°C to -80°C. Reconstituted: Use immediately or aliquot and store at -20°C to -80°C. Avoid freeze/thaw cycles.
Dichiarazione di non responsabilità
Questo prodotto è solo per uso di ricerca. Non è inteso per uso diagnostico o terapeutico.
Flow Cytometry - Recombinant Phl p 7 (7.0101) Protein (A242915) - Antibodies.comClicca sull'immagine per la visualizzazione estesa
Figure 1: Flow Cytometry - Recombinant Phl p 7 (7.0101) Protein (A242916)
Flow cytometry dot-plot staining pattern of Recombinant Phl p 7 (7.0101) Protein stimulated human peripheral whole blood lymphocytes and basophils of a proven allergic donor stained using Anti-CD63 [MEM-259] (FITC) Antibody and Anti-CD203c [NP4D6] (PE) Antibody.
SDS-PAGE - Recombinant Phl p 7 (7.0101) Protein (A242916) - Antibodies.comClicca sull'immagine per la visualizzazione estesa
Figure 2: SDS-PAGE - Recombinant Phl p 7 (7.0101) Protein (A242916)
Purity verification: 5 µg of Recombinant Phl p 7 (7.0101) Protein with > 95 % purity checked by Coomassie Brilliant Blue stained SDS-PAGE.
ELISA - Recombinant Phl p 7 (7.0101) Protein (A242916) - Antibodies.comClicca sull'immagine per la visualizzazione estesa
Figure 3: Recombinant Phl p 7 (7.0101) Protein (A242916)
An ELISA test was designed to prove the bond between the coated target, Recombinant Phl p 7 (7.0101) Protein, and allergen-specific human plasma IgG4 antibodies of Phleum pratense positive donor. A measurable signal was subsequently generated by the addition of Anti-Human IgG4 Antibody (Biotin), Streptavidin-HRP, and substrate solution (TMB). The intensity of the signal is proportional to the amount of coated Recombinant Phl p 7 (7.0101) Protein.
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