Anti-RNF7 Anticorpo [ARC3065] (A309459)

Name: Anti-RNF7 Antibody [ARC3065]
Brand: Antibodies.com
SKU: A309459

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Questo prodotto è fuori produzione

Anti-RNF7 Anticorpo [ARC3065] (A309459) è stato interrotto e non è più disponibile.

Visualizza tutti gli Anti-RNF7 Anticorpi.

Descrizione Prodotto

Nome

Anti-RNF7 Antibody [ARC3065]

Descrizione

Rabbit monoclonal [ARC3065] antibody to RNF7.

Applicazioni

WB, IHC

Diluizioni consigliate

WB: 1:500-1:1,000, IHC: 1:50-1:200

Reattività

Human, Mouse, Rat

Immunogeno

Recombinant protein of Human RNF7.

Specie ospite

Rabbit

Clonalità

Monoclonal

Clona ID

ARC3065

Isotipo

IgG

Coniugare

Unconjugated

Purificazione

Affinity purification.

Peso molecolare

13 kDa

Forma del prodotto

Liquid

Formulazione

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.02% Sodium Azide.

Conservazione

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Sinonimi

CKBBP 1, CKBBP1, CKII beta binding protein 1, CKII beta-binding protein 1, Rbx 2, Rbx2, RBX2_HUMAN, Regulator of cullins 2, RING box protein 2, RING finger protein 7, RING-box protein 2, RNF 7, ROC 2, ROC2, SAG, Sensitive to apoptosis gene, Sensitive to apoptosis gene protein, Zinc RING finger protein SAG

Controlli isotipo

Secondari adatti

Dichiarazione di non responsabilità

Questo prodotto è solo per uso di ricerca. Non è inteso per uso diagnostico o terapeutico.

Immagini (5)

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Figure 1: Western Blot - Anti-RNF7 Antibody [ARC3065] (A309459)

Western blot analysis of extracts of various cell lines, using Anti-RNF7 Antibody [ARC3065] (A309459) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 180s.

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Figure 2: Western Blot - Anti-RNF7 Antibody [ARC3065] (A309459)

Western blot analysis of extracts of DU145 cells, using Anti-RNF7 Antibody [ARC3065] (A309459) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Enhanced Kit (RM00021). Exposure time: 90s.

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Figure 3: Immunohistochemistry - Anti-RNF7 Antibody [ARC3065] (A309459)

Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue using Anti-RNF7 Antibody [ARC3065] (A309459) at a dilution of 1:100(40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Figure 4: Immunohistochemistry - Anti-RNF7 Antibody [ARC3065] (A309459)

Immunohistochemistry analysis of paraffin-embedded mouse spinal cord using Anti-RNF7 Antibody [ARC3065] (A309459) at a dilution of 1:100(40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.