Anti-S6K1 (phospho Thr389) Anticorpo (A305998)

Name: Anti-S6K1 (phospho Thr389) Antibody
Brand: Antibodies.com
SKU: A305998
Price: 435.0 USD
Availability: InStock

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$435

Anticorpo policlonale di coniglio contro S6K1 (phospho Thr389) per WB e IHC.

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Consegna

Spese di spedizione

$40

Tempi di consegna

5-8 Giorni lavorativi

Contattaci

Telefono

+1 (314) 370-6046

E-mail

orders@antibodies.com

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Tag dei prodotti:

Ribosomal Marker

Descrizione Prodotto

Nome

Anti-S6K1 (phospho Thr389) Antibody

Descrizione

Rabbit polyclonal antibody to S6K1 (phospho Thr389).

Applicazioni

WB, IHC

Diluizioni consigliate

WB: 1:500-1:2,000, IHC: 1:50-1:200

Reattività

Human, Mouse, Rat

Immunogeno

A synthetic phosphorylated peptide around T389 of human P70S6K1 (NP_001258989.1?).

Sequenza

GFTYV

Specie ospite

Rabbit

Clonalità

Polyclonal

Isotipo

IgG

Coniugare

Unconjugated

Purificazione

Affinity purification.

Peso molecolare

70 kDa / 85 kDa

Forma del prodotto

Liquid

Formulazione

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.01% Thiomersal.

Conservazione

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Sinonimi

70 kDa ribosomal protein S6 kinase 1, KS6B1_HUMAN, p70 alpha, P70 beta 1, p70 ribosomal S6 kinase alpha, p70 ribosomal S6 kinase beta 1, P70 S6 Kinase, p70 S6 kinase alpha, p70 S6 kinase, alpha 1, p70 S6 kinase, alpha 2, p70 S6K, p70 S6K-alpha, p70 S6KA, p70(S6K) alpha, p70(S6K)-alpha, p70-alpha, p70-S6K, p70-S6K 1, P70S6K, P70S6K1, p70S6Kb, PS6K, Ribosomal protein S6 kinase 70kDa polypeptide 1, Ribosomal protein S6 kinase beta 1, Ribosomal protein S6 kinase beta-1, Ribosomal protein S6 kinase I, RPS6KB1, S6K, S6K-beta-1, Serine/threonine kinase 14 alpha, Serine/threonine-protein kinase 14A, STK14A

Controlli isotipo

Secondari adatti

Dichiarazione di non responsabilità

Questo prodotto è solo per uso di ricerca. Non è inteso per uso diagnostico o terapeutico.

Immagini (6)

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Figure 1: Western Blot - Anti-S6K1 (phospho Thr389) Antibody (A305998)

Western blot analysis of NIH/3T3, using Anti-S6K1 (phospho Thr389) Antibody (A305998) at 1:500 dilution. NIH/3T3 cells were treated by 10% FBS at 37°C for 30 minutes after serum-starvation overnight. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 90s.

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Figure 2: Western Blot - Anti-S6K1 (phospho Thr389) Antibody (A305998)

Western blot analysis of C6, using Anti-S6K1 (phospho Thr389) Antibody (A305998) at 1:500 dilution. C6 cells were treated by Î»-PP mixed solution (1ul) at 30°C for 30 minutes. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 90s.

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Figure 3: Immunohistochemistry - Anti-S6K1 (phospho Thr389) Antibody (A305998)

Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue using Anti-S6K1 (phospho Thr389) Antibody (A305998) at a dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Figure 4: Immunohistochemistry - Anti-S6K1 (phospho Thr389) Antibody (A305998)

Immunohistochemistry analysis of paraffin-embedded human colon tissue using Anti-S6K1 (phospho Thr389) Antibody (A305998) at a dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.