Anti-Smad1 Anticorpo (A309166)

Name: Anti-Smad1 Antibody
Brand: Antibodies.com
SKU: A309166
Price: 470.0 USD
Availability: InStock

Clicca sull'immagine per la visualizzazione estesa

$470

Anticorpo policlonale di coniglio contro Smad1 per WB, IHC e ICC/IF.

Garanzia al 100%

Garanzia di corrispondenza del prezzo

Consegna

Spese di spedizione

$40

Tempi di consegna

5-8 Giorni lavorativi

Contattaci

Telefono

+1 (314) 370-6046

E-mail

orders@antibodies.com

Garanzie

Garanzia di qualità al 100%

Garanzia di corrispondenza del prezzo

Tag dei prodotti:

Anticorpo Knockout Convalidato

Descrizione Prodotto

Nome

Anti-Smad1 Antibody

Descrizione

Rabbit polyclonal antibody to Smad1.

Applicazioni

WB, IHC, ICC/IF

Diluizioni consigliate

WB: 1:500-1:1,000, IHC: 1:50-1:200, ICC/IF: 1:50-1:200

Reattività

Human, Mouse, Rat

Immunogeno

Recombinant fusion protein containing a sequence corresponding to amino acids 20-240 of human Smad1 (NP_005891.1).

Sequenza

WKQGDEEEKWAEKAVDALVKKLKKKKGAMEELEKALSCPGQPSNCVTIPRSLDGRLQVSHRKGLPHVIYCRVWRWPDLQSHHELKPLECCEFPFGSKQKEVCINPYHYKRVESPVLPPVLVPRHSEYNPQHSLLAQFRNLGQNEPHMPLNATFPDSFQQPNSHPFPHSPNSSYPNSPGSSSSTYPHSPTSSDPGSPFQMPADTPPPAYLPPEDPMTQDGSQ

Specie ospite

Rabbit

Clonalità

Polyclonal

Isotipo

IgG

Coniugare

Unconjugated

Purificazione

Affinity purification.

Peso molecolare

60 kDa

Forma del prodotto

Liquid

Formulazione

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.05% Proclin 300.

Conservazione

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Sinonimi

BSP-1, BSP1, HsMAD1, JV4-1, JV41, MAD homolog 1, MAD mothers against decapentaplegic homolog 1, Mad related protein 1, Mad-related protein 1, MADH1, MADR1, Mothers against decapentaplegic homolog 1, Mothers against DPP homolog 1, SMA- AND MAD-RELATED PROTEIN 1, SMAD 1, SMAD family member 1, SMAD mothers against DPP homolog 1, SMAD1_HUMAN, TGF beta signaling protein 1, Transforming growth factor-beta-signaling protein 1

Controlli isotipo

Secondari adatti

Dichiarazione di non responsabilità

Questo prodotto è solo per uso di ricerca. Non è inteso per uso diagnostico o terapeutico.

Immagini (6)

Clicca sull'immagine per la visualizzazione estesa

Figure 1: Western Blot - Anti-Smad1 Antibody (A309166)

Western blot analysis of extracts of various cell lines, using Anti-Smad1 Antibody (A309166) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 10s.

Clicca sull'immagine per la visualizzazione estesa

Figure 2: Western Blot - Anti-Smad1 Antibody (A309166)

Western blot analysis of extracts from wild type (WT) and Smad1 knockout (KO) HeLa cells, using Anti-Smad1 Antibody (A309166) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 10s.

Clicca sull'immagine per la visualizzazione estesa

Figure 3: Immunohistochemistry - Anti-Smad1 Antibody (A309166)

Immunohistochemistry analysis of paraffin-embedded human breast cancer tissue using Anti-Smad1 Antibody (A309166) at a dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Clicca sull'immagine per la visualizzazione estesa

Figure 4: Immunohistochemistry - Anti-Smad1 Antibody (A309166)

Immunohistochemistry analysis of paraffin-embedded mouse lung using Anti-Smad1 Antibody (A309166) at a dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.