Anti-Smad2 Anticorpo [ARC0343] (A307856)

Name: Anti-Smad2 Antibody [ARC0343]
Brand: Antibodies.com
SKU: A307856
Price: 470.0 USD
Availability: InStock

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$470

Anticorpo monoclonale [ARC0343] di coniglio contro Smad2 per WB, ICC/IF e IP.

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Consegna

Spese di spedizione

$40

Tempi di consegna

5-8 Giorni lavorativi

Contattaci

Telefono

+1 (314) 370-6046

E-mail

orders@antibodies.com

Garanzie

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Descrizione Prodotto

Nome

Anti-Smad2 Antibody [ARC0343]

Descrizione

Rabbit monoclonal [ARC0343] antibody to Smad2.

Applicazioni

WB, ICC/IF, IP

Diluizioni consigliate

WB: 1:500-1:2,000, ICC/IF: 1:50-1:200, IP: 1:500-1:1,000

Reattività

Human, Mouse, Rat

Immunogeno

A synthetic peptide corresponding to a sequence within amino acids 1-100 of human Smad2 (Q15796).

Sequenza

MSSILPFTPPVVKRLLGWKKSAGGSGGAGGGEQNGQEEKWCEKAVKSLVKKLKKTGRLDELEKAITTQNCNTKCVTIPSTCSEIWGLSTPNTIDQWDTTG

Specie ospite

Rabbit

Clonalità

Monoclonal

Clona ID

ARC0343

Isotipo

IgG

Coniugare

Unconjugated

Purificazione

Affinity purification.

Peso molecolare

58 kDa

Forma del prodotto

Liquid

Formulazione

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.02% Sodium Azide.

Conservazione

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Sinonimi

Drosophila, homolog of, MADR2, hMAD-2, HsMAD2, JV18, JV18-1, JV181, MAD, MAD homolog 2, MAD Related Protein 2, Mad-related protein 2, MADH2, MADR2, MGC22139, MGC34440, Mother against DPP homolog 2, Mothers against decapentaplegic homolog 2, Mothers against decapentaplegic, Drosophila, homolog of, 2, Mothers against DPP homolog 2, OTTHUMP00000163489, Sma and Mad related protein 2, Sma- and Mad-related protein 2 MAD, SMAD 2, SMAD family member 2, SMAD, mothers against DPP homolog 2, SMAD2_HUMAN

Controlli isotipo

Secondari adatti

Dichiarazione di non responsabilità

Questo prodotto è solo per uso di ricerca. Non è inteso per uso diagnostico o terapeutico.

Immagini (5)

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Figure 1: Western Blot - Anti-Smad2 Antibody [ARC0343] (A307856)

Western blot analysis of extracts from wild type (WT) and Smad2 knockdown (KD) HeLa cells, using Anti-Smad2 Antibody [ARC0343] (A307856) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 90s.

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Figure 2: Western Blot - Anti-Smad2 Antibody [ARC0343] (A307856)

Western blot analysis of extracts of various cell lines, using Anti-Smad2 Antibody [ARC0343] (A307856) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 90s.

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Figure 3: Western Blot - Anti-Smad2 Antibody [ARC0343] (A307856)

Western blot analysis of extracts of Rat lung, using Anti-Smad2 Antibody [ARC0343] (A307856) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 3min.

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Figure 4: Immunofluorescence - Anti-Smad2 Antibody [ARC0343] (A307856)

Immunofluorescence analysis of NIH-3T3 cells using Anti-Smad2 Antibody [ARC0343] (A307856) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).