Anti-Sonic Hedgehog Anticorpo (A16913)

Name: Anti-Sonic Hedgehog Antibody
Brand: Antibodies.com
SKU: A16913
Price: 335.0 USD
Availability: InStock

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$335

Anticorpo policlonale di coniglio contro Sonic Hedgehog per WB, IHC e ICC/IF.

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Consegna

Spese di spedizione

$40

Tempi di consegna

5-8 Giorni lavorativi

Contattaci

Telefono

+1 (314) 370-6046

E-mail

orders@antibodies.com

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Descrizione Prodotto

Nome

Anti-Sonic Hedgehog Antibody

Descrizione

Rabbit polyclonal antibody to Sonic Hedgehog.

Applicazioni

WB, IHC, ICC/IF

Diluizioni consigliate

WB: 1:500-1:1,000, IHC: 1:50-1:100, ICC/IF: 1:50-1:200

Reattività

Human, Mouse, Rat

Immunogeno

A synthetic peptide corresponding to a sequence within amino acids 200-300 of human Sonic Hedgehog (Shh) (NP_000184.1).

Sequenza

PGSATVHLEQGGTKLVKDLSPGDRVLAADDQGRLLYSDFLTFLDRDDGAKKVFYVIETREPRERLLLTAAHLLFVAPHNDSATGEPEASSGSGPPSGGALG

Specie ospite

Rabbit

Clonalità

Polyclonal

Isotipo

IgG

Coniugare

Unconjugated

Purificazione

Affinity purification.

Peso molecolare

19 kDa

Forma del prodotto

Liquid

Formulazione

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.05% Proclin 300.

Conservazione

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Sinonimi

HHG 1, HHG-1, HHG1, HLP 3, HLP3, Holoprosencephaly 3, HPE 3, HPE3, MCOPCB5, shh, SHH_HUMAN, SMMC I, SMMCI, Sonic Hedgehog (Drosophila) homolog, Sonic hedgehog homolog, sonic hedgehog homolog (Drosophila), Sonic hedgehog protein, Sonic hedgehog protein C-product, TPT, TPTPS

Controlli isotipo

Secondari adatti

Dichiarazione di non responsabilità

Questo prodotto è solo per uso di ricerca. Non è inteso per uso diagnostico o terapeutico.

Immagini (6)

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Figure 1: Western Blot - Anti-Sonic Hedgehog Antibody (A16913)

Western blot analysis of extracts of various cell lines, using Anti-Sonic Hedgehog Antibody (A16913) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 180s.

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Figure 2: Western Blot - Anti-Sonic Hedgehog Antibody (A16913)

Western blot analysis of extracts of Rat lung, using Anti-Sonic Hedgehog Antibody (A16913) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Enhanced Kit (RM00021). Exposure time: 180s.

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Figure 3: Immunohistochemistry - Anti-Sonic Hedgehog Antibody (A16913)

Immunohistochemistry analysis of paraffin-embedded rat kidney using Anti-Sonic Hedgehog Antibody (A16913) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

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Figure 4: Immunohistochemistry - Anti-Sonic Hedgehog Antibody (A16913)

Immunohistochemistry analysis of paraffin-embedded rat lung using Anti-Sonic Hedgehog Antibody (A16913) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.