Anti-STAT5 (phospho Tyr694) Anticorpo [ARC0176] (A305577)

Name: Anti-STAT5 (phospho Tyr694) Antibody [ARC0176]
Brand: Antibodies.com
SKU: A305577

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Questo prodotto è fuori produzione

Anti-STAT5 (phospho Tyr694) Anticorpo [ARC0176] (A305577) è stato interrotto e non è più disponibile.

Visualizza tutti gli Anti-STAT5 Anticorpi.

Descrizione Prodotto

Nome

Anti-STAT5 (phospho Tyr694) Antibody [ARC0176]

Descrizione

Rabbit monoclonal [ARC0176] antibody to STAT5 (phospho Tyr694).

Applicazioni

WB, IHC

Diluizioni consigliate

WB: 1:500-1:2,000, IHC: 1:50-1:200

Reattività

Human, Mouse, Rat

Immunogeno

A synthetic phosphorylated peptide around Y694 of human Stat5 (P42229).

Sequenza

DGYVK

Specie ospite

Rabbit

Clonalità

Monoclonal

Clona ID

ARC0176

Isotipo

IgG

Coniugare

Unconjugated

Purificazione

Affinity purification.

Peso molecolare

100 kDa

Forma del prodotto

Liquid

Formulazione

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.02% Sodium Azide.

Conservazione

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Sinonimi

MGF, Signal transducer and activator of transcription 5A, Signal Transducer and Activator of Transcription 5B, STA5A_HUMAN, STAT 5A, STAT 5B, STAT5A, STAT5B, Transcription factor STAT5A, Transcription factor STAT5B

Controlli isotipo

Secondari adatti

Dichiarazione di non responsabilità

Questo prodotto è solo per uso di ricerca. Non è inteso per uso diagnostico o terapeutico.

Immagini (4)

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Figure 1: Western Blot - Anti-STAT5 (phospho Tyr694) Antibody [ARC0176] (A305577)

Western blot analysis of TF-1 cells, using Anti-STAT5 (phospho Tyr694) Antibody [ARC0176] (A305577) at 1:1,000 dilution. TF-1 cells were treated by GM-CSF (25 ng/ml) at 37°C for 30 minutes after serum-starvation overnight. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 60s.

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Figure 2: Immunohistochemistry - Anti-STAT5 (phospho Tyr694) Antibody [ARC0176] (A305577)

Immunohistochemistry analysis of paraffin-embedded rat brain using Anti-STAT5 (phospho Tyr694) Antibody [ARC0176] (A305577) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

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Figure 3: Immunohistochemistry - Anti-STAT5 (phospho Tyr694) Antibody [ARC0176] (A305577)

Immunohistochemistry analysis of paraffin-embedded human oophoroma using Anti-STAT5 (phospho Tyr694) Antibody [ARC0176] (A305577) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

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Figure 4: Immunohistochemistry - Anti-STAT5 (phospho Tyr694) Antibody [ARC0176] (A305577)

Immunohistochemistry analysis of paraffin-embedded mouse kidney using Anti-STAT5 (phospho Tyr694) Antibody [ARC0176] (A305577) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.