This Superoxide Anion Assay Kit is a quantitative assay kit for measuring Superoxide Anion in serum, plasma, animal and plant tissues, cells, and cell supernatant.

Applicazioni

Functional Studies

Principio di kit

The superoxide anion in plant, animal tissues, serum and other samples reacts with hydroxylamine hydrochloride to produce NO2- , which then reacts with Gris reagent to generate a red azo compound with a characteristic absorption peak at 540 nm. The O2- content in the sample can be calculated based on the A540 value. The kit can detect samples of plants, animal tissues, serum, and cells.

Tipo di campione

Serum, plasma, animal tissues, plant tissues, cells, and cell supernatant.

Componenti

Extraction Buffer, Reagent I, Reagent II, Reagent III, NaNO₂ Standard (10 mmoL/L)

Conservazione

Shipped at 4°C. Store at 4°C in the dark. Refer to the Protocol Manual for storage conditions of individual components. Do not use past expiration date!

Note generali

96-well microplate / microglass cuvette is required.

Dichiarazione di non responsabilità

Questo prodotto è solo per uso di ricerca. Non è inteso per uso diagnostico o terapeutico.

Immagini (1)

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Figure 1: Standard Curve - Superoxide Anion Assay Kit (A319739)

Representative standard curve for Superoxide Anion Assay Kit (A319739).The x-axis is OD540 and the y-axis is NO2- concentration (µM).

Consegna

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Garanzie

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