Synthetic Membrane Nanoparticle Human Adenosine Receptor A2a Protein
Descrizione
Full-length transmembrane human Adenosine Receptor A2a protein expressed on the cell surface of membrane nanoparticles.
Applicazioni
ELISA, FACS
Sistema di espressione
HEK293 cells
Tipo di proteina
Synthetic
Specie proteiche
Human
Lunghezza
Full length protein.
Peso molecolare
Full length human Adenosine Receptor A2a protein has a MW of 44.7 kDa.
Coniugare
Unconjugated
Forma del prodotto
Lyophilized
Concentrazione
Reconstitution dependent.
Formulazione
Lyophilized from sterile Phosphate Buffered Saline, pH 7.4. Normally 5%–8% Trehalose is added as a protectant before lyophilization.
Conservazione
Shipped at 4°C. Lyophilized: Store at -20°C to -80°C. Reconstituted: Aliquot and store at -80°C. Product is stable for one year. Avoid freeze/thaw cycles.
Figure 1: Synthetic Membrane Nanoparticle Human Adenosine Receptor A2a Protein (A318479)
ELISA plates were pre-coated with 0.5 µg/per well Synthetic Membrane Nanoparticle Human Adenosine Receptor A2a Protein (A318479). Serial diluted Anti-Flag Tag Antibody solutions were added, washed, and incubated with secondary antibody before ELISA reading. From above data, the EC50 for Anti-Flag Tag Antibody binding with CLDN6 full length membrane nanoparticles is 6.796 µg/ml.
Figure 2: Synthetic Membrane Nanoparticle Human Adenosine Receptor A2a Protein (A318479)
FACS analysis of Synthetic Membrane Nanoparticle Human Adenosine Receptor A2a Protein (A318479): A. Negative Control 1: Synthetic Membrane Nanoparticle Human Adenosine Receptor A2a Protein (A318479) samples were stained only with Goat Anti-Mouse lgG Antibody (Alexa Fluor 488). B. Negative Control 2: Control membrane nanoparticles samples were stained with Anti-ADORA2A Antibody at 2 µg/ml, followed by Goat Anti-Mouse lgG Antibody (Alexa Fluor 488). C. Negative Control 3: Synthetic Membrane Nanoparticle Human Adenosine Receptor A2a Protein (A318479) samples were stained with Anti-His Tag Antibody (an irrelevant antibody) at 2 µg/ml, followed by Goat Anti-Mouse lgG Antibody (Alexa Fluor 488). D. Synthetic Membrane Nanoparticle Human Adenosine Receptor A2a Protein (A318479) samples were stained with Anti-ADORA2A Antibody at 2 µg/ml, followed by Goat Anti-Mouse lgG Antibody (Alexa Fluor 488).
