Synthetic Membrane Nanoparticle Human Cannabinoid Receptor I Protein
Descrizione
Full-length transmembrane human Cannabinoid Receptor I protein expressed on the cell surface of membrane nanoparticles.
Applicazioni
ELISA, FACS
Sistema di espressione
HEK293 cells
Tipo di proteina
Synthetic
Specie proteiche
Human
Lunghezza
Full length protein.
Peso molecolare
Full length human Cannabinoid Receptor I protein has a MW of 52.7 kDa.
Coniugare
Unconjugated
Forma del prodotto
Lyophilized
Concentrazione
Reconstitution dependent.
Formulazione
Lyophilized from sterile Phosphate Buffered Saline, pH 7.4. Normally 5%–8% Trehalose is added as a protectant before lyophilization.
Conservazione
Shipped at 4°C. Lyophilized: Store at -20°C to -80°C. Reconstituted: Aliquot and store at -80°C. Product is stable for one year. Avoid freeze/thaw cycles.
Figure 1: Synthetic Membrane Nanoparticle Human Cannabinoid Receptor I Protein (A318488)
ELISA plates were pre-coated with 0.5 µg/per well Synthetic Membrane Nanoparticle Human Cannabinoid Receptor I Protein (A318488). Serial diluted Anti-Cannabinoid Receptor I Antibody [DM144] - Azide free (A318564) solutions were added, washed, and incubated with secondary antibody before ELISA reading. From above data, the EC50 for Anti-Cannabinoid Receptor I Antibody [DM144] - Azide free (A318564) binding with CB1 full length membrane nanoparticles is 439.6 µg/ml.
Figure 2: Synthetic Membrane Nanoparticle Human Cannabinoid Receptor I Protein (A318488)
FACS analysis of Synthetic Membrane Nanoparticle Human Cannabinoid Receptor I Protein (A318488): A. Negative Control 1: Synthetic Membrane Nanoparticle Human Cannabinoid Receptor I Protein (A318488) samples were stained only with Goat Anti-Human lgG Antibody (Alexa Fluor 488). B. Negative Control 2: Control membrane nanoparticles samples were stained with Anti-Cannabinoid Receptor I Humanized Antibody [Nimacimab Biosimilar] - Azide free (A318908) at 2 µg/ml, followed by Goat Anti-Human lgG Antibody (Alexa Fluor 488). C. Negative Control 3: Synthetic Membrane Nanoparticle Human Cannabinoid Receptor I Protein (A318488) samples were stained with Anti-CCR8 Antibody (an irrelevant antibody) at 2 µg/ml, followed by Goat Anti-Human lgG Antibody (Alexa Fluor 488). D. Synthetic Membrane Nanoparticle Human Cannabinoid Receptor I Protein (A318488) samples were stained with Anti-Cannabinoid Receptor I Humanized Antibody [Nimacimab Biosimilar] - Azide free (A318908) at 2 µg/ml, followed by Goat Anti-Human lgG Antibody (Alexa Fluor 488).
Prodotti alternativi a Sintetico Membrane Nanoparticle Umano Cannabinoid Receptor I proteinaa (A318488)
