Synthetic Membrane Nanoparticle Human Claudin 6 Protein
Descrizione
Full-length transmembrane human Claudin 6 protein expressed on the cell surface of membrane nanoparticles.
Applicazioni
ELISA, FACS
Sistema di espressione
HEK293 cells
Tipo di proteina
Synthetic
Specie proteiche
Human
Lunghezza
Full length protein.
Peso molecolare
Full length human Claudin 6 protein has a MW of 23 kDa.
Coniugare
Unconjugated
Forma del prodotto
Lyophilized
Concentrazione
Reconstitution dependent.
Formulazione
Lyophilized from sterile Phosphate Buffered Saline, pH 7.4. Normally 5%–8% Trehalose is added as a protectant before lyophilization.
Conservazione
Shipped at 4°C. Lyophilized: Store at -20°C to -80°C. Reconstituted: Aliquot and store at -80°C. Product is stable for one year. Avoid freeze/thaw cycles.
Figure 1: Synthetic Membrane Nanoparticle Human Claudin 6 Protein (A318491)
ELISA plates were pre-coated with 0.5 µg/per well Synthetic Membrane Nanoparticle Human Claudin 6 Protein (A318491). Serial diluted Anti-Claudin 6 Humanized Antibody [IMAB027] - Azide free (A318880) solutions were added, washed, and incubated with secondary antibody before ELISA reading. From above data, the EC50 for Anti-Claudin 6 Humanized Antibody [IMAB027] - Azide free (A318880) binding with CLDN6 full length membrane nanoparticles is 34.36 µg/ml.
Figure 2: Synthetic Membrane Nanoparticle Human Claudin 6 Protein (A318491)
FACS analysis of Synthetic Membrane Nanoparticle Human Claudin 6 Protein (A318491): A. Negative Control 1: Synthetic Membrane Nanoparticle Human Claudin 6 Protein (A318491) samples were stained only with Goat Anti-Human lgG Antibody (Alexa Fluor 488). B. Negative Control 2: Control membrane nanoparticles samples were stained with Anti-Claudin 6 Humanized Antibody [IMAB027] - Azide free (A318880) at 2 µg/ml, followed by Goat Anti-Human lgG Antibody (Alexa Fluor 488). C. Negative Control 3: Synthetic Membrane Nanoparticle Human Claudin 6 Protein (A318491) samples were stained with Anti-GPRC5D Antibody (an irrelevant antibody) at 2 µg/ml, followed by Goat Anti-Human lgG Antibody (Alexa Fluor 488). D. Synthetic Membrane Nanoparticle Human Claudin 6 Protein (A318491) samples were stained with Anti-Claudin 6 Humanized Antibody [IMAB027] - Azide free (A318880) at 2 µg/ml, followed by Goat Anti-Human lgG Antibody (Alexa Fluor 488).
