Figure 1: Western Blot - Anti-Thrombomodulin Antibody [ARC52599 + ARC52600] (A309567)
Western blot analysis of extracts of A-431, using Anti-Thrombomodulin Antibody [ARC52599 + ARC52600] (A309567) at 1:5,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 1s.
Immunohistochemistry analysis of paraffin-embedded human lung squamous carcinoma tissue using Anti-Thrombomodulin Antibody [ARC52599 + ARC52600] (A309567) at a dilution of 1:500 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry analysis of paraffin-embedded human lung using Anti-Thrombomodulin Antibody [ARC52599 + ARC52600] (A309567) at a dilution of 1:500 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry analysis of paraffin-embedded human tonsil using Anti-Thrombomodulin Antibody [ARC52599 + ARC52600] (A309567) at a dilution of 1:500 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunofluorescence analysis of A431 using Anti-Thrombomodulin Antibody [ARC52599 + ARC52600] (A309567) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).
Immunofluorescence analysis of THP-1 using Anti-Thrombomodulin Antibody [ARC52599 + ARC52600] (A309567) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).
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