Anti-TRPC5 Anticorpo [N67/15] (A304954)

Name: Anti-TRPC5 Antibody [N67/15]
Brand: Antibodies.com
SKU: A304954
Price: 500.0 USD
Availability: InStock

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$500

Anticorpo monoclonale [N67/15] di topo contro TRPC5 per WB, IHC, IP e Antibody Microarray.

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Consegna

Spese di spedizione

$40

Tempi di consegna

5-8 Giorni lavorativi

Contattaci

Telefono

+1 (314) 370-6046

E-mail

orders@antibodies.com

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Descrizione Prodotto

Nome

Anti-TRPC5 Antibody [N67/15]

Descrizione

Mouse monoclonal [N67/15] antibody to TRPC5.

Applicazioni

WB, IHC, IP, Antibody Microarray

Diluizioni consigliate

WB: 1:1,000, IHC: 1:1,000, ICC/IF: 1:100

Reattività

Human, Mouse, Rat

Immunogeno

Synthetic peptide amino acids 827-845 of human TrpC5 (also known as short transient receptor potential channel 5, and Htrp5).

Specie ospite

Mouse

Clonalità

Monoclonal

Clona ID

N67/15

Isotipo

IgG2b

Coniugare

Unconjugated

Purificazione

Protein G purification.

Concentrazione

1 mg/ml

Peso molecolare

~110 kDa

Forma del prodotto

Liquid

Formulazione

Supplied in Phosphate Buffered Saline, pH 7.4, with 50% Glycerol and 0.09% Sodium Azide.

Conservazione

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Note generali

This antibody was formely sold under the Clone ID S67-15.

Sinonimi

Htrp 5, Htrp-5, Htrp5, Short transient receptor potential channel 5, Transient receptor potential cation channel subfamily C member 5, Transient receptor potential channel 5, Transient receptor protein 5, TRP 5, TRP-5, TRP5, TRPC 5, TRPC5_HUMAN

Controlli isotipo

Secondari adatti

Dichiarazione di non responsabilità

Questo prodotto è solo per uso di ricerca. Non è inteso per uso diagnostico o terapeutico.

Immagini (3)

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Figure 1: Western Blot - Anti-TRPC5 Antibody [N67/15] (A304954)

Western blot analysis of mouse brain showing detection of 110 kDa TrpC5 protein using Anti-TRPC5 Antibody [N67/15] (A304954) at 1:1,000 for Overnight at 4°C. Lane 1: Molecular Weight Ladder (MW). Lane 2: mouse Brain. Load: 15 ug. Block: 5% Skim Milk powder in TBST. The secondary antibody used was Goat anti-mouse IgG:HRP at 1:7000 for 1 hour at room temperature with shaking. Color Development: Chemiluminescent for HRP (Moss) for 5 minutes in room temperature. Predicted/Observed Size: 110 kDa.

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Figure 2: Immunohistochemistry - Anti-TRPC5 Antibody [N67/15] (A304954)

Immunohistochemistry analysis of mouse brain slice, fixed in 10% formalin solution for 12-24 hours at room temperature. The Primary Antibody used was Anti-TRPC5 Antibody [N67/15] (A304954) at 1:1,000 for 1 hour at room temperature. The secondary antibody used was HRP/DAB Detection System: Biotinylated Goat Anti-Mouse, Streptavidin Peroxidase, DAB Chromogen (brown) for 30 minutes at room temperature. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 250-500 µl for 5 minutes at room temperature. Localization: Nuclear staining. Magnification: 10X.

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Figure 3: Immunofluorescence - Anti-TRPC5 Antibody [N67/15] (A304954)

Immunocytochemistry/Immunofluorescence analysis of human neuroblastoma cells (SH-SY5Y), fixed in 4% PFA for 15 min, using Anti-TRPC5 Antibody [N67/15] (A304954), at 1:50 for overnight at 4°C with slow rocking. The secondary antibody used was AlexaFluor 488 at 1:1,000 for 1 hour at room temperature. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 minutes at room temperature.(A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) TrpC5 Antibody (D) Composite.