Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.02% Sodium Azide.
Conservazione
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Sinonimi
General vesicular transport factor, General vesicular transport factor p115, P115, Protein USO1 homolog, TAP, Transcytosis associated protein, Transcytosis-associated protein, USO1_HUMAN, VDP, Vesicle docking protein, Vesicle docking protein p115, Vesicle-docking protein
Figure 1: Western Blot - Anti-USO1 Antibody [ARC2937] (A307551)
Western blot analysis of extracts of various cell lines, using Anti-USO1 Antibody [ARC2937] (A307551) at 1:500 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 1s.
Immunohistochemistry analysis of paraffin-embedded human lung cancer using Anti-USO1 Antibody [ARC2937] (A307551) at a dilution of 1:20 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry analysis of paraffin-embedded mouse kidney using Anti-USO1 Antibody [ARC2937] (A307551) at a dilution of 1:20 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry analysis of paraffin-embedded rat brain using Anti-USO1 Antibody [ARC2937] (A307551) at a dilution of 1:20 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Confocal imaging of HeLa cells using Anti-USO1 Antibody [ARC2937] (A307551), at a dilution of 1:100, (red). The cells were counterstained with Anti-alpha Tubulin Antibody, at a dilution of 1:200, (green). DAPI was used for nuclear staining (Blue). Objective: 60x.