Figure 1: Western Blot - Anti-Vimentin Antibody (A81195)
Western blot analysis of extracts of various cell lines, using Anti-Vimentin Antibody (A81195) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 10s.
Figure 2: Western Blot - Anti-Vimentin Antibody (A81195)
Western blot analysis of extracts from normal (control) and Vimentin knockout (KO) 293T cells, using Anti-Vimentin Antibody (A81195) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 5s.
Immunofluorescence analysis of U2OS cells using Anti-Vimentin Antibody (A81195) at a dilution of 1:50 (40x lens). DAPI was used to stain the cell nuclei (blue).
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