Anti-VPS15 Anticorpo (A305142)

Name: Anti-VPS15 Antibody
Brand: Antibodies.com
SKU: A305142
Price: 500.0 USD
Availability: InStock

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$500

Anticorpo policlonale di coniglio contro VPS15 per WB e ICC/IF.

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Consegna

Spese di spedizione

$40

Tempi di consegna

5-8 Giorni lavorativi

Contattaci

Telefono

+1 (314) 370-6046

E-mail

orders@antibodies.com

Garanzie

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Descrizione Prodotto

Nome

Anti-VPS15 Antibody

Descrizione

Rabbit polyclonal antibody to VPS15.

Applicazioni

WB, ICC/IF

Diluizioni consigliate

WB: 1:1,000, ICC/IF: 1:100

Reattività

Human, Rat

Immunogeno

Synthetic peptide from the C-terminal of Human PIK3R4 (aa. 1314-1325).

Specie ospite

Rabbit

Clonalità

Polyclonal

Isotipo

IgG

Coniugare

Unconjugated

Purificazione

Peptide affinity purification.

Concentrazione

1 mg/ml

Peso molecolare

~160 kDa

Forma del prodotto

Liquid

Formulazione

Supplied in Phosphate Buffered Saline with 50% Glycerol and 0.09% Sodium Azide.

Conservazione

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Sinonimi

MGC102700, P150, Phosphatidylinositol 3 kinase associated p150, Phosphatidylinositol 3 kinase regulatory subunit polypeptide 4, Phosphatidylinositol 3 kinase regulatory subunit polypeptide 4 p150, Phosphoinositide 3 kinase adaptor protein, Phosphoinositide 3 kinase regulatory subunit 4, Phosphoinositide 3 kinase regulatory subunit 4 p150, Phosphoinositide 3 kinase regulatory subunit polypeptide 4, Phosphoinositide 3 kinase regulatory subunit polypeptide 4 p150, Phosphoinositide 3-kinase adaptor protein, Phosphoinositide 3-kinase regulatory subunit 4, PI 3 Kinase regulatory subunit 4, PI3 kinase p150 subunit, PI3-kinase p150 subunit, PI3-kinase regulatory subunit 4, PI3R4_HUMAN, PIK3R 4, PIK3R4, VPS 15

Controlli isotipo

Secondari adatti

Dichiarazione di non responsabilità

Questo prodotto è solo per uso di ricerca. Non è inteso per uso diagnostico o terapeutico.

Immagini (2)

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Figure 1: Immunofluorescence - Anti-VPS15 Antibody (A305142)

Immunocytochemistry/Immunofluorescence analysis of human neuroblastoma cell line (SK-N-BE, fixed in 4% formaldehyde for 15 min at room temperature, using Anti-VPS15 Antibody (A305142), at 1:100 for 60 minutes at room temperature. The secondary antibody used was Goat Anti-Rabbit ATTO 488 at 1:200 for 60 minutes at room temperature. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5,000 for 60 minutes at room temperature, 5 minutes at room temperature. Localization: Endosome, Cytoplasmic Vesicle, Autophagosome, Membrane, Lipid-Anchor. Magnification: 60X.(A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) PIK3R4 Antibody (D) Composite.

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Figure 2: Western Blot - Anti-VPS15 Antibody (A305142)

Western blot analysis of rat brain cell lysates showing detection of 153.1 kDa PIK3R4 protein using Anti-VPS15 Antibody (A305142) at 1:1,000 for 1 hour at room temperature. Lane 1: Molecular Weight Ladder (MW). Lane 2: rat brain cell lysates. Load: 15 µg. Block: 5% Skim Milk in 1X TBST. The secondary antibody used was Goat Anti-Rabbit HRP at 1:2000 for 60 minutes at room temperature. Color Development: ECL solution for 6 min in room temperature. Predicted/Observed Size: 153.1 kDa.