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Anti-Mad-1 Antibody

Rabbit polyclonal antibody to Mad-1 from ABclonal (A0648).
Name: Anti-Mad-1 Antibody
Description: Rabbit polyclonal antibody to Mad-1.
Applications: WB
Dilutions: WB: 1:500 - 1:1000.
Reactivity: Human
Immunogen: A synthetic peptide of human MAD1L1.
Protein Length: 718
Host: Rabbit
Clonality: Polyclonal
Isotype: IgG
Conjugate: Unconjugated
Purification: Affinity purification.
Product Form: Liquid
Formulation: Supplied in Phosphate Buffered Saline, pH 7.30, with 0.02% Sodium Azide and 50% Glycerol.
Storage: Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Function: Component of the spindle-assembly checkpoint that prevents the onset of anaphase until all chromosomes are properly aligned at the metaphase plate. May recruit MAD2L1 to unattached kinetochores. Has a role in the correct positioning of the septum. Required for anchoring MAD2L1 to the nuclear periphery. Binds to the TERT promoter and represses telomerase expression, possibly by interfering with MYC binding.
Tissue Specificity: Expressed weakly at G0/G1 and highly at late S and G2/M phase.
Sequence Similarities: Belongs to the MAD1 family.
Post-Translational Modification: Phosphorylated; by BUB1. Become hyperphosphorylated in late S through M phases or after mitotic spindle damage.
Cellular Location: Nucleus. Chromosome > Centromere > Kinetochore. Cytoplasm > Cytoskeleton > Microtubule organizing center > Centrosome. Cytoplasm > Cytoskeleton > Spindle.

From the beginning to the end of mitosis, it is seen to move from a diffusely nuclear distribution to the centrosome, to the spindle midzone and finally to the midbody. Colocalizes with NEK2 at the kinetochore.
UniProt: Q9Y6D9
Gene ID: 8379
Western Blot - Anti-Mad-1 Antibody from ABclonal (A0648) - Antibodies.com
Western blot analysis of extracts of A431 cells tissue, using MAD1L1 antibody.
Please Note: All batches are extensively validated and are guaranteed to work as specified, however, QC data is not necessarily from the current lot on sale.
Please Note: Anti-Mad-1 Antibody is for research use only. It is not intended for diagnostic or therapeutic use.
Antibodies.com | A0648
Size Price Quantity
100µl £243
200µl £372

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Lead Time: Currently 4-6 Days
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  • £20 to United Kingdom
  • £35 to Europe