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Anti-mCherry Antibody

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Total Citations (28)
Rabbit polyclonal antibody to mCherry from EnCor Biotechnology (RPCA-mCherry).

   Please Note: This is exactly the same antibody that is marketed as:


 
   •  Abcam | ab167453
Name: Anti-mCherry Antibody
Description: Rabbit polyclonal antibody to mCherry.
Applications: WB, ICC/IF, IHC
Dilutions: WB: 1:1,000. IF/IHC: 1:500.
Immunogen: Full length recombinant protein.
Host: Rabbit
Clonality: Polyclonal
Isotype: IgG
Conjugate: Unconjugated
Purification: Immunogen affinity purified.
Concentration: 1 mg/ml
Molecular Weight: ~28 kDa
Product Form: Liquid
Storage: Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Introduction: mCherry is derived from proteins originally isolated from Cnidarians (jelly fish, sea anemones and corals), and is used as a fluorescent tracer in trasfection and transgenic experiments. The prototype for these fluorescent proteins is Green Fluorescent Protein (GFP), which is a ~27 kDa protein isolated originally from the jellyfish Aequoria victoria. GFP was the basis of the 2008 Nobel Prize in Chemistry, awarded to Osamu Shimomura, Martin Chalfie and Roger Tsien, specifically “for the discovery and development of the green fluorescent protein, GFP”. GFP was shown to fluoresce on contact with molecular oxygen, requiring no other cofactors, and so can be expressed in fluorescent form in essentially any prokaryotic or eukaryotic cell. The mCherry protein is derived from DsRed, a red fluorescent protein related to GFP isolated from so-called disc corals of the genus Discosoma. DsRed is similar in size and properties to GFP, but, obviously, produces a red rather than a green fluorochrome. The original DsRed was engineered extensively in the Tsien lab to prevent it from forming tetramers and dimers and to modify and improve the spectral properties (1-3). Several further cycles of mutation, directed modification and evolutionary selection produced mCherry, which has an excitation maximum at 587 nm and and emission maximum at 610 nm (4). We expressed the mCherry protein sequence shown in reference 4 in bacteria, purified out the mCherry and raised this rabbit polyclonal antibody. This was affinity purified and was found to stain a band of the expected size in HEK293 cells transfected with the pFin-EF1-mCherry vector designed to express mCherry which was obtained from Clontech. As shown below, the antibody does not stain any protein band in untransfected HEK293 cells.
Immunohistochemisty - Anti-mCherry Antibody from EnCor Biotechnology (RPCA-mCherry) - Antibodies.com
HEK293 cells transfected in the same way and viewed using a confocal microscope. Most HEK293 cells are not transfected so only the nucleus of these cells can be visualized with a blue DNA stain. Cells which are transfected with mCherry are red. Staining with RPCA-mCherry is shown in Green. Green antibody staining is only seen in cells which express mCherry, as expected, and the superimposition of the green and red signals results in an orange signal. Interestingly, stronger mCherry staining is seen in the nucleus, possibly due to degradation of some mCherry molecules to release the low molecular weight mCherry fluorochrome. Blot and transfected cells courtesy of the Semple-Rowland lab at the University of Florida.
Western Blot - Anti-mCherry Antibody from EnCor Biotechnology (RPCA-mCherry) - Antibodies.com
Blot of HEK293 cells transfected with pFin-EF1-mCherry vector, in the lane marked “+”. HEK293 cells which were not transfected with this vector show no protein band in lane marked “-“.
Please Note: All batches are extensively validated and are guaranteed to work as specified, however, QC data is not necessarily from the current lot on sale.

   Please Note: These publications cite this antibody under different brand names & catalogue numbers.


Cites ab167453

Ramat A  et al. Maintenance of Miranda Localization in Drosophila Neuroblasts Involves Interaction with the Cognate mRNA. Curr Biol 27:2101-2111.e5 (2017).

Cites ab167453

Lim NR  et al. Glial-Specific Functions of Microcephaly Protein WDR62 and Interaction with the Mitotic Kinase AURKA Are Essential for Drosophila Brain Growth. Stem Cell Reports 9:32-41 (2017).

Cites ab167453

Verboogen DRJ  et al. Fluorescence Lifetime Imaging Microscopy reveals rerouting of SNARE trafficking driving dendritic cell activation. Elife 6:N/A (2017).

Cites ab167453

Howe JR  et al. MiR-338-3p regulates neuronal maturation and suppresses glioblastoma proliferation. PLoS One 12:e0177661 (2017).

Cites ab167453

Parenti N  et al. Direct imaging of APP proteolysis in living cells. PeerJ 5:e3086 (2017).

Cites ab167453

Huang Y  et al. Critical roles of CTP synthase N-terminal in cytoophidium assembly. Exp Cell Res 354:122-133 (2017).

Cites ab167453

Bressan RB  et al. Efficient CRISPR/Cas9-assisted gene targeting enables rapid and precise genetic manipulation of mammalian neural stem cells. Development 144:635-648 (2017).

Cites ab167453

Manfredi-Lozano M  et al. Defining a novel leptin-melanocortin-kisspeptin pathway involved in the metabolic control of puberty. Mol Metab 5:844-57 (2016).

Cites ab167453

Beloate LN  et al. Ventral Tegmental Area Dopamine Cell Activation during Male Rat Sexual Behavior Regulates Neuroplasticity and d-Amphetamine Cross-Sensitization following Sex Abstinence. J Neurosci 36:9949-61 (2016).

Cites ab167453

Qiu J  et al. High-frequency stimulation-induced peptide release synchronizes arcuate kisspeptin neurons and excites GnRH neurons. Elife 5:N/A (2016).

Cites ab167453

Akanuma T  et al. Memory of cell shape biases stochastic fate decision-making despite mitotic rounding. Nat Commun 7:11963 (2016).

Cites ab167453

Kühn A  et al. The IRX1/HOXA connection: insights into a novel t(4;11)- specific cancer mechanism. Oncotarget 7:35341-52 (2016).

Cites ab167453

Winkle CC  et al. Trim9 Deletion Alters the Morphogenesis of Developing and Adult-Born Hippocampal Neurons and Impairs Spatial Learning and Memory. J Neurosci 36:4940-58 (2016).

Cites ab167453

Liu SY  et al. A small molecule induces integrin ß4 nuclear translocation and apoptosis selectively in cancer cells with high expression of integrin ß4. Oncotarget 7:16282-96 (2016).

Cites ab167453

Morozova N  et al. Temporal dynamics of methyltransferase and restriction endonuclease accumulation in individual cells after introducing a restriction-modification system. Nucleic Acids Res 44:790-800 (2016).

Cites ab167453

Kim J  et al. PLK1 regulation of PCNT cleavage ensures fidelity of centriole separation during mitotic exit. Nat Commun 6:10076 (2015).

Cites ab167453

Thukral L  et al. The Molecular Mechanism Underlying Recruitment and Insertion of Lipid-Anchored LC3 Protein into Membranes. Biophys J 109:2067-78 (2015).

Cites ab167453

Sluch VM  et al. Differentiation of human ESCs to retinal ganglion cells using a CRISPR engineered reporter cell line. Sci Rep 5:16595 (2015).

Cites ab167453

Whissell PD  et al. Comparative density of CCK- and PV-GABA cells within the cortex and hippocampus. Front Neuroanat 9:124 (2015).

Cites ab167453

Jovasevic V  et al. GABAergic mechanisms regulated by miR-33 encode state-dependent fear. Nat Neurosci 18:1265-71 (2015).

Cites ab167453

Idevall-Hagren O  et al. Triggered Ca2+ influx is required for extended synaptotagmin 1-induced ER-plasma membrane tethering. EMBO J 34:2291-305 (2015).

Cites ab167453

Breznau EB  et al. MgcRacGAP restricts active RhoA at the cytokinetic furrow and both RhoA and Rac1 at cell-cell junctions in epithelial cells. Mol Biol Cell 26:2439-55 (2015).

Cites ab167453

Erdmann F  et al. Neuronal expression of the human neuropeptide S receptor NPSR1 identifies NPS-induced calcium signaling pathways. PLoS One 10:e0117319 (2015).

Cites ab167453

Duellman T  et al. Quantitation of secreted proteins using mCherry fusion constructs and a fluorescent microplate reader. Anal Biochem 473:34-40 (2015).

Cites ab167453

Ashour J  et al. Intracellular expression of camelid single-domain antibodies specific for influenza virus nucleoprotein uncovers distinct features of its nuclear localization. J Virol 89:2792-800 (2015).

Cites ab167453

Luo X & He JJ Cell-cell contact viral transfer contributes to HIV infection and persistence in astrocytes. J Neurovirol 21:66-80 (2015).

Cites ab167453

Nie X  et al. The HIRA complex that deposits the histone H3.3 is conserved in Arabidopsis and facilitates transcriptional dynamics. Biol Open 3:794-802 (2014).

Cites ab167453

Lee TK  et al. A dynamically assembled cell wall synthesis machinery buffers cell growth. Proc Natl Acad Sci U S A 111:4554-9 (2014).

Shaner NC, Campbell RE, Steinbach PA, Giepmans BN, Palmer AE, Tsien RY. Improved monomeric red, orange and yellow fluorescent proteins derived from Discosoma sp. red fluorescent protein. Nature Biotechnology 22:1567-1572 (2004)

Heikal AA, Hess ST, Baird GS, Tsien RY, Webb WW. Molecular spectroscopy and dynamics of intrinsically fluorescent proteins: coral red (dsRed) and yellow (Citrine). Proc Natl Acad Sci U S A. 97:11996-2001 (2000)

Gross LA, Baird GS, Hoffman RC, Baldridge KK, Tsien RY. The structure of the chromophore within DsRed, a red fluorescent protein from coral. Proc Natl Acad Sci U S A. 97:11990-5 (2000)

Baird GS, Zacharias DA, Tsien RY. Biochemistry, mutagenesis, and oligomerization of DsRed, a red fluorescent protein from coral. Proc Natl Acad Sci U S A. 97:11984-9 (2000)

Rating

Species

Application

Date

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Mouse

IHC-P

15-Nov-2017

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Human

ICC/IF

24-Apr-2017

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Mouse

IHC

24-Jan-2017

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Human

ICC/IF

30-Aug-2016

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Mouse

IHC-P

03-Jun-2016

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Mouse

IHC-P

12-May-2015

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Human

IHC-Fr

20-Apr-2015

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Human

WB

11-Feb-2015

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Mouse

ICC/IF

04-Mar-2014

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Mouse

IHC-Fr

25-Feb-2014


start icons start icons start icons start icons start icons (8)
Total Citations (35)
Description: Mouse monoclonal (1C51) antibody to mCherry.
Application: WB, ICC/IF, IHC
Host: Mouse
Isotype: IgG2a
Immunogen: Full length recombinant protein.

£165 – £714

Description: Chicken polyclonal antibody to mCherry.
Application: WB, ICC/IF, IHC
Host: Chicken
Isotype: IgY
Immunogen: Full length recombinant protein.

£165 – £714

Description: Mouse monoclonal antibody to mCherry
Application: WB, IP
Host: Mouse

£180 – £234

Description: Mouse monoclonal (Clone 1) antibody to mCherry.
Application: WB
Host: Mouse
Isotype: IgG1
Reactivity: All

£295





Please Note: Anti-mCherry Antibody is for research use only. It is not intended for diagnostic or therapeutic use.
Antibodies.com | RPCA-mCherry
Size Price Quantity
50µl £189
100µl £270
500µl £875

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