+44 (0)1223 298875

Anti-NF-H Antibody

start icons start icons start icons start icons start icons (8)
Total Citations (26)
Chicken polyclonal antibody to NF-H from EnCor Biotechnology (CPCA-NF-H).

   Please Note: This is exactly the same antibody that is marketed as:


 
   •  Abcam | ab4680
Name: Anti-NF-H Antibody
Description: Chicken polyclonal antibody to NF-H.
Applications: WB, ICC/IF, IHC
Dilutions: WB: 1:1,000,000. ICC/IF and IHC: 1:100,000.
Immunogen: Native NF-H purified from bovine spinal cord.
Host: Chicken
Clonality: Polyclonal
Isotype: IgY
Conjugate: Unconjugated
Molecular Weight: 200 kDa
Purity: IgY preparation.
Product Form: Liquid
Storage: Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Introduction: Neurofilaments can be defined as the intermediate or 10nm filaments found in specifically in neuronal cells. In the electron microscope neurofilaments appears as 10nm diameter fibers of indeterminate length which generally have fine wispy protrusions from their sides. They are particularly abundan in axons of large projection neurons. They probably function to provide structural support for neurons and their synapses and to support the large axon diameters required for rapid conduction of impulses down axons. They are composed a mixture of subunits which usually include the three neurofilament triplet proteins, known as NF-L, NF-M and NF-H. Neurofilaments may also include smaller amounts of peripherin, alpha-internexin, nestin and in some cases vimentin. Antibodies to the various neurofilament subunits are very useful cell type markers since the proteins are among the most abundant in the nervous system, are expressed only in neurons, and are biochemically very stable. The characterization of this antibody has been published in peer-reviewed form and has been shown to be very effective in ELISA (1). The antibody has a very high titer and can be used in immunofluoresence experiments at dilutions of 1 to 1 million, and reacts primarily with the phosphorylated axonal form of NF-H. As with many antibodies to NF-H, this antibody has some cross-reactivity with phosphorylated NF-M, which has similar phosphorylation sites to NF-H. To raise this particular antibody bovine intermediate filaments were prepared from spinal cords by the glycerol polymerization method of Delacourte et al., and the cytoskeletal material was dissolved in 6M urea (2). Individual neurofilament subunits were purified by ion exchange chromatography on DEAE-cellulose and the NF-H containing fractions were concentrated and further purified by preparative gel electrophoresis on a Biorad Prepcell. The HGNC name for this protein is NEFH. This antibody was generated in chicken by standard procedures and immunoglobulin was extracted from egg yolk. The resulting polyclonal antibody belongs to the IgY subclass. This is the chicken homologue of mammalian IgG and can be used in the same general way, with the caveat that this type of antibody does not bind either Protein A or Protein G.
Immunohistochemisty - Anti-NF-H Antibody from EnCor Biotechnology (CPCA-NF-H) - Antibodies.com
Mixed neuron/glial cultures stained with mouse monoclonal antibody to neurofilament subunit NF-L, MCA-7D1, (green) and CPCA-NF-H, EnCor’s rabbit antibody to neurofilament NF-H. This antibody binds primarily to the phosphorylated axonal forms of NF-H, in contrast to the NF-L antibody which stains both axonal and dendritic/perikaryal neurofilaments. The NF-L antibody therefore reveals a prominent cell body in green, while the surrounding axonal profiles are orange, since the are bound by both NF-L and the chicken NF-H antibody. Blue is a DNA stain.
Immunohistochemisty - Anti-NF-H Antibody from EnCor Biotechnology (CPCA-NF-H) - Antibodies.com
Lane labelled CBB shows Coomassie Brilliant Blue stained crude extract of rat spinal cord, with the prominent major neurofilament subunit indicated; In rodents, NF-H runs at 200kDa, NF-M at 145kDa and NF-L at 68kDa. The Rab lane shows a blot probed with rabbit anti NF-H antibody RPCA-NF-H. A prominent band at an apparent SDS-PAGE molecular weight of 200kDa corresponds to phosphorylated form of NF-H. The lane marked Rab shows a similar blot probed with our rabbit antibody to NF-H, RPCA-NF-H.
Please Note: All batches are extensively validated and are guaranteed to work as specified, however, QC data is not necessarily from the current lot on sale.

Pre- and postsynaptic changes in the neuromuscular junction in dystrophic mice.

M2 microglia and macrophages drive oligodendrocyte differentiation during CNS remyelination.

Expansion of neurofilament medium C terminus increases axonal diameter independent of increases in conduction velocity or myelin thickness.

Muscle pathology without severe nerve pathology in a new mouse model of Charcot-Marie-Tooth disease type 2E.

Maintenance of axo-oligodendroglial paranodal junctions requires DCC and netrin-1.

   Please Note: These publications cite this antibody under different brand names & catalogue numbers.


Cites ab4680

Carr L  et al. Expression patterns of Slit and Robo family members in adult mouse spinal cord and peripheral nervous system. PLoS One 12:e0172736 (2017).

Cites ab4680

Esanov R  et al. The FMR1 promoter is selectively hydroxymethylated in primary neurons of fragile X syndrome patients. Hum Mol Genet 25:4870-4880 (2016).

Cites ab4680

Szczyrba J  et al. Neuroendocrine Cells of the Prostate Derive from the Neural Crest. J Biol Chem 292:2021-2031 (2017).

Cites ab4680

Hu G  et al. Single-cell RNA-seq reveals distinct injury responses in different types of DRG sensory neurons. Sci Rep 6:31851 (2016).

Cites ab4680

Barzan R  et al. N- and L-Type Voltage-Gated Calcium Channels Mediate Fast Calcium Transients in Axonal Shafts of Mouse Peripheral Nerve. Front Cell Neurosci 10:135 (2016).

Cites ab4680

Cabral CM  et al. Neurons are the Primary Target Cell for the Brain-Tropic Intracellular Parasite Toxoplasma gondii. PLoS Pathog 12:e1005447 (2016).

Cites ab4680

Buniello A  et al. Wbp2 is required for normal glutamatergic synapses in the cochlea and is crucial for hearing. EMBO Mol Med 8:191-207 (2016).

Cites ab4680

Ford MC  et al. Tuning of Ranvier node and internode properties in myelinated axons to adjust action potential timing. Nat Commun 6:8073 (2015).

Cites ab4680

Dun XP & Parkinson DB Visualizing peripheral nerve regeneration by whole mount staining. PLoS One 10:e0119168 (2015).

Cites ab4680

Patra K  et al. A role for solute carrier family 10 member 4, or vesicular aminergic-associated transporter, in structural remodelling and transmitter release at the mouse neuromuscular junction. Eur J Neurosci 41:316-27 (2015).

Cites ab4680

Maksimovic S  et al. Epidermal Merkel cells are mechanosensory cells that tune mammalian touch receptors. Nature 509:617-21 (2014).

Cites ab4680

Kranz K  et al. Phosphodiesterase type 4 inhibitor rolipram improves survival of spiral ganglion neurons in vitro. PLoS One 9:e92157 (2014).

Cites ab4680

Osório C  et al. Selective regulation of axonal growth from developing hippocampal neurons by tumor necrosis factor superfamily member APRIL. Mol Cell Neurosci 59:24-36 (2014).

Cites ab4680

Belugin S  et al. Mechanisms of transient signaling via short and long prolactin receptor isoforms in female and male sensory neurons. J Biol Chem 288:34943-55 (2013).

Cites ab4680

Couve E  et al. Mitochondrial autophagy and lipofuscin accumulation in aging odontoblasts. J Dent Res 91:696-701 (2012).

Cites ab4680

Sparrow N  et al. The actin-severing protein cofilin is downstream of neuregulin signaling and is essential for Schwann cell myelination. J Neurosci 32:5284-97 (2012).

Cites ab4680

Watson RP  et al. Increased prokineticin 2 expression in gut inflammation: role in visceral pain and intestinal ion transport. Neurogastroenterol Motil 24:65-75, e12 (2012).

Cites ab4680

Diogenes A  et al. LPS sensitizes TRPV1 via activation of TLR4 in trigeminal sensory neurons. J Dent Res 90:759-64 (2011).

Cites ab4680

Wirt SE  et al. G1 arrest and differentiation can occur independently of Rb family function. J Cell Biol 191:809-25 (2010).

Cites ab4680

Zeng F  et al. Glial cell-derived neurotrophic factor enhances synaptic communication and 5-hydroxytryptamine 3a receptor expression in enteric neurons. Gastroenterology 138:1491-501 (2010).

Cites ab4680

Wells JE  et al. Kv1.4 subunit expression is decreased in neurons of painful human pulp. J Endod 33:827-9 (2007).

Delacourte A, Filliatreau G, Boutteau F, Biserte G, Schrevel J. Study of the 10-nm- filament fraction isolated during the standard microtubule preparation. Biochem J. 191:543-6 (1980).

Shaw G, Yang C, Ellis R, Anderson K, Parker Mickle J, Scheff S, Pike B, Anderson DK and Howland DR. Hyperphosphorylated neurofilament NF-H is a serum biomarker of axonal injury. Biochem Biophys Res Commun. 336:1268-1277 (2005).

Rating

Species

Application

Date

start icons start icons start icons start icons start icons

Human

ICC

17-Feb-2016

start icons start icons start icons start icons start icons

Porcine

ICC/IF

20-Mar-2015

start icons start icons start icons start icons start icons

Rat

IHC-Fr

16-Mar-2015

start icons start icons start icons start icons start icons

Rat

IHC-Fr

30-Apr-2014

start icons start icons start icons start icons start icons

Aplysia

IHC-Fr

30-Sep-2013

start icons start icons start icons start icons start icons

Mouse

IHC

05-Jul-2012

start icons start icons start icons start icons start icons

Mouse

IHC-Fr

29-May-2012

start icons start icons start icons start icons start icons

Rat

IHC-Fr

21-Aug-2009


Total Citations (1)
Description: Mouse monoclonal (NAP4) antibody to NF-H.
Application: WB, ICC/IF, IHC
Host: Mouse
Isotype: IgG1
Immunogen: Native NF-H purified from bovine spinal cord.

£189 – £875

Description: Rabbit polyclonal antibody to NF-H (E972)
Application: WB, IHC
Host: Rabbit
Reactivity: Human, Mouse, Rat
Immunogen: Synthetic peptide, corresponding to amino acids 941-990 of Human NF-H.

£165 – £283

Description: Mouse monoclonal (9B12) antibody to NF-H.
Application: WB, ICC/IF, IHC
Host: Mouse
Isotype: IgG2b
Immunogen: Native NF-H purified from bovine spinal cord.

£189 – £875

Description: Rabbit polyclonal antibody to NF-H.
Application: WB, ICC/IF, IHC
Host: Rabbit
Isotype: IgG
Immunogen: Native NF-H purified from bovine spinal cord.

£165 – £714

Please Note: Anti-NF-H Antibody is for research use only. It is not intended for diagnostic or therapeutic use.
Antibodies.com | CPCA-NF-H
Size Price Quantity
25µl £189
50µl £270
100µl £472
250µl £875

or
Request a quotation
Contact Us Directly
  • call icon + 44 (0)1223 298 875
  • + 44 (0)1223 281 447
  • email icon info@antibodies.com
Product Quality
  • guarantee icon
  • We promise guaranteed product quality
  • and expert customer service.
Lead Time: Currently 3-4 Days
Shipping Information
  • £20 to United Kingdom
  • £35 to Europe