Anti-SMC1A (phospho Ser957) Antibody (A10885)

Name: Anti-SMC1A (phospho Ser957) Antibody
Brand: Antibodies.com
SKU: A10885
Price: 435.0 USD
Availability: InStock

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$435

Rabbit polyclonal antibody to SMC1A (phospho Ser957) for WB, IHC and IP.

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Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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+1 (314) 370-6046

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Specifications

Name

Anti-SMC1A (phospho Ser957) Antibody

Description

Rabbit polyclonal antibody to SMC1A (phospho Ser957).

Applications

WB, IHC, IP

Dilutions

WB: 1:500-1:2,000, IHC: 1:50-1:200, IP: 1:500-1:1,000

Reactivity

Human, Mouse, Rat

Immunogen

A synthetic phosphorylated peptide around S957 of human SMC1A (NP_006297.2).

Sequence

GSSQG

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

160 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

Chromosome segregation protein SmcB, DXS423E, KIAA0178, MGC138332, Sb1.8, Segregation of mitotic chromosomes 1, SMC protein 1A, SMC-1-alpha, SMC-1A, SMC1, SMC1 (structural maintenance of chromosomes 1 yeast) like 1, SMC1 structural maintenance of chromosomes 1 like 1, SMC1alpha, SMC1A_HUMAN, SMC1L1, SMCB, Structural maintenance of chromosomes 1A, Structural maintenance of chromosomes protein 1A

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (4)

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Figure 1: Western Blot - Anti-SMC1A (phospho Ser957) Antibody (A10885)

Western blot analysis of extracts of various cell lines, using Anti-SMC1A (phospho Ser957) Antibody (A10885) at 1:1,000 dilution. HeLa and NIH/3T3 cells were treated by UV for 15-30 minutes. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% BSA. Detection was with a ECL Basic Kit. Exposure time: 3s.

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Figure 2: Western Blot - Anti-SMC1A (phospho Ser957) Antibody (A10885)

Western blot analysis of extracts of HeLa cells, using Anti-SMC1A (phospho Ser957) Antibody (A10885) at 1:1,000 dilution or SMC1A antibody (A7008). HeLa cells were treated by UV at room temperature for 15-30 minutes. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% BSA. Detection was with a ECL Basic Kit. Exposure time: 3s.

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Figure 3: Immunohistochemistry - Anti-SMC1A (phospho Ser957) Antibody (A10885)

Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue using Anti-SMC1A (phospho Ser957) Antibody (A10885) at a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Figure 4: Western Blot - Anti-SMC1A (phospho Ser957) Antibody (A10885)

Immunoprecipitation analysis of 300µg extracts of HeLa cells using 3µg of Anti-SMC1A (phospho Ser957) Antibody (A10885). This Western blot was performed on the immunoprecipitate using Anti-SMC1A (phospho Ser957) Antibody (A10885) at a dilution of 1:1000.

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