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Anti-STAT1 Antibody

Mouse monoclonal antibody to STAT1 from ABclonal (A10100).
Name: Anti-STAT1 Antibody
Description: Mouse monoclonal antibody to STAT1.
Applications: WB, IP
Dilutions: WB: 1:500 - 1:2000, IP: 1:20 - 1:50.
Reactivity: Human
Immunogen: Recombinant protein of human STAT1.
Protein Length: 750 (712)
Host: Mouse
Clonality: Monoclonal
Isotype: IgG
Conjugate: Unconjugated
Purification: Affinity purification.
Product Form: Liquid
Formulation: Supplied in Phosphate Buffered Saline, pH 7.30, with 0.02% Sodium Azide and 50% Glycerol.
Storage: Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Function: Signal transducer and transcription activator that mediates cellular responses to interferons (IFNs), cytokine KITLG/SCF and other cytokines and other growth factors. Following type I IFN (IFN-alpha and IFN-beta) binding to cell surface receptors, signaling via protein kinases leads to activation of Jak kinases (TYK2 and JAK1) and to tyrosine phosphorylation of STAT1 and STAT2. The phosphorylated STATs dimerize and associate with ISGF3G/IRF-9 to form a complex termed ISGF3 transcription factor, that enters the nucleus. ISGF3 binds to the IFN stimulated response element (ISRE) to activate the transcription of IFN-stimulated genes (ISG), which drive the cell in an antiviral state. In response to type II IFN (IFN-gamma), STAT1 is tyrosine- and serine-phosphorylated. It then forms a homodimer termed IFN-gamma-activated factor (GAF), migrates into the nucleus and binds to the IFN gamma activated sequence (GAS) to drive the expression of the target genes, inducing a cellular antiviral state. Becomes activated in response to KITLG/SCF and KIT signaling. May mediate cellular responses to activated FGFR1, FGFR2, FGFR3 and FGFR4.
Involvement in Disease: Immunodeficiency 31B: A disorder characterized by susceptibility to severe mycobacterial and viral infections. Affected individuals can develop disseminated infections and die of viral illness.

Immunodeficiency 31A: A form of Mendelian susceptibility to mycobacterial disease, a rare condition caused by impairment of interferon-gamma mediated immunity. It is characterized by predisposition to illness caused by moderately virulent mycobacterial species, such as Bacillus Calmette-Guerin (BCG) vaccine, environmental non-tuberculous mycobacteria, and by the more virulent Mycobacterium tuberculosis. Other microorganisms rarely cause severe clinical disease in individuals with susceptibility to mycobacterial infections, with the exception of Salmonella which infects less than 50% of these individuals. Clinical outcome severity depends on the degree of impairment of interferon-gamma mediated immunity. Some patients die of overwhelming mycobacterial disease with lepromatous-like lesions in early childhood, whereas others develop, later in life, disseminated but curable infections with tuberculoid granulomas. IMD31A has low penetrance, and affected individuals have relatively mild disease and good prognosis. IMD31A confers a predisposition to mycobacterial infections only, with no increased susceptibility to viral infections.

Immunodeficiency 31C: A primary immunodeficiency disorder with altered immune responses and impaired clearance of fungal infections, selective against Candida. It is characterized by persistent and/or recurrent infections of the skin, nails and mucous membranes caused by organisms of the genus Candida, mainly Candida albicans.
Sequence Similarities: Belongs to the transcription factor STAT family.
Post-Translational Modification: Phosphorylated on tyrosine and serine residues in response to a variety of cytokines/growth hormones including IFN-alpha, IFN-gamma, PDGF and EGF. Activated KIT promotes phosphorylation on tyrosine residues and subsequent translocation to the nucleus. Upon EGF stimulation, phosphorylation on Tyr-701 (lacking in beta form) by JAK1, JAK2 or TYK2 promotes dimerization and subsequent translocation to the nucleus. Growth hormone (GH) activates STAT1 signaling only via JAK2. Tyrosine phosphorylated in response to constitutively activated FGFR1, FGFR2, FGFR3 and FGFR4. Phosphorylation on Ser-727 by several kinases including MAPK14, ERK1/2 and CAMKII on IFN-gamma stimulation, regulates STAT1 transcriptional activity. Phosphorylation on Ser-727 promotes sumoylation though increasing interaction with PIAS. Phosphorylation on Ser-727 by PRKCD induces apoptosis in response to DNA-damaging agents. Phosphorylated on tyrosine residues when PTK2/FAK1 is activated; most likely this is catalyzed by a SRC family kinase. Dephosphorylation on tyrosine residues by PTPN2 negatively regulates interferon-mediated signaling. Upon viral infection or IFN induction, phosphorylation on Ser-708 occurs much later than phosphorylation on Tyr-701 and is required for the binding of ISGF3 on the ISREs of a subset of IFN-stimulated genes IKBKE-dependent. Phosphorylation at Tyr-701 and Ser-708 are mutually exclusive, phosphorylation at Ser-708 requires previous dephosphorylation of Tyr-701.
Cellular Location: Cytoplasm. Nucleus.

Translocated into the nucleus upon tyrosine phosphorylation and dimerization, in response to IFN-gamma and signaling by activated FGFR1, FGFR2, FGFR3 or FGFR4.
UniProt: P42224
Gene ID: 6772
Synonyms: Signal transducer and activator of transcription 1 91kD Antibody
CANDF7 Antibody
DKFZp686B04100 Antibody
IMD31A Antibody
IMD31B Antibody
IMD31C Antibody
ISGF 3 Antibody
ISGF-3 Antibody
ISGF3 Antibody
OTTHUMP00000163552 Antibody
OTTHUMP00000165046 Antibody
OTTHUMP00000165047 Antibody
OTTHUMP00000205845 Antibody
Signal transducer and activator of transcription 1 Antibody
Signal transducer and activator of transcription 1 91kDa Antibody
Signal transducer and activator of transcription 1, 91kD Antibody
Signal transducer and activator of transcription 1, 91kDa Antibody
Signal transducer and activator of transcription 1-alpha/beta Antibody
STAT 1 Antibody
STAT1 alpha Antibody
STAT1_HUMAN Antibody
STAT91 Antibody
Transcription factor ISGF 3 components p91 p84 Antibody
Transcription factor ISGF-3 components p91/p84 Antibody
Transcription factor ISGF3 components p91/p84 Antibody
XStat1 Antibody
Information: Target information shown above is from the UniProt Consortium.
Western blot analysis of extracts of various cells, using STAT1 antibody.
Please Note: All batches are extensively validated and are guaranteed to work as specified, however, QC data is not necessarily from the current lot on sale.
Total Citations (7)
Description: Rabbit polyclonal antibody to Stat1
Application: WB, IHC
Host: Rabbit
Reactivity: Human, Mouse, Rat
Immunogen: Synthetic peptide, corresponding to amino acids 680-720 of Human Stat1.

£165 – £283

start icons start icons start icons start icons start icons (3)
Total Citations (7)
Description: Mouse monoclonal (SM1) antibody to STAT1.
Application: IP, WB, IHC-P
Host: Mouse
Isotype: IgG2b
Reactivity: Mouse, Human

£224





Total Citations (4)
Description: Rabbit polyclonal antibody to Stat1 (G695)
Application: WB, IHC, IP
Host: Rabbit
Reactivity: Human, Mouse, Rat
Immunogen: Synthetic peptide, corresponding to amino acids 680-720 of Human Stat1.

£165 – £283

Total Citations (4)
Description: Rabbit polyclonal antibody to STAT1 (Phospho-Tyr701)
Application: WB, IHC
Host: Rabbit
Reactivity: Human, Mouse
Immunogen: Peptide sequence around phosphorylation site of tyrosine 701 (T-G-Y(p)-I-K) derived from Human STAT1.

£180 – £234

Please Note: Anti-STAT1 Antibody is for research use only. It is not intended for diagnostic or therapeutic use.
Antibodies.com | A10100
Size Price Quantity
50µl £199
100µl £307
200µl £452

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