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Anti-Ubiquitin Antibody

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Total Citations (33)
Mouse monoclonal (Ubi-1) antibody to Ubiquitin from EnCor Biotechnology (MCA-Ubi-1).

   Please Note: This is exactly the same antibody that is marketed as:


 
   •  Abcam | ab7254
Name: Anti-Ubiquitin Antibody
Description: Mouse monoclonal (Ubi-1) antibody to Ubiquitin.
Applications: WB, ICC/IF, IHC
Dilutions: WB: 1:1,000. IF/ICC and IHC: 1:1,000.
Reactivity: Human, Horse, Cow, Pig, Chicken, Rat, Mouse, Drosophila, C. elegans
Immunogen: Purified ubiquitin conjugated with glutaraldehyde to KLH.
Host: Mouse
Clonality: Monoclonal
Clone: Ubi-1
Isotype: IgG1
Conjugate: Unconjugated
Purification: Immunogen affinity purified.
Concentration: 1 mg/ml
Molecular Weight: 8.5 kDa
Product Form: Liquid
Storage: Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Introduction: Ubiquitin is a highly conserved globular 76 amino acid protein of about 8.5 kDa molecular weight. It has a important role in the targeting of proteins for proteolytic degradation. Proteins to be degraded are covalently coupled to the C-terminus of ubiquitin by means of ubiquitin ligases. The ubiquitin itself is frequently also ubiquitinated, producing a polyubiquitin chain. The polyubquitinated complex is then recognized by a complex of degradative enzymes which together form the proteosome. Interestingly, ubiquitin also becomes covalently bonded to many types of pathological inclusions seen in serious human disease states which appear to be resistant to normal degradation, so that ubiquitin antibodies are very useful for studies of these inclusions. For example the neurofibrillary tangles and paired helical filaments diagnostic of Alzheimer’s disease, the Lewy bodies seen in Parkinson’s disease, and Pick bodies found in Pick’s disease are all heavily ubiquitinated and can all be readily visualized with ubiquitin antibodies of appropriate specificity. MCA-Ubi-1 was made in the University of Florida in 1987, and has been continuously on the market place since 1989, and is now being sold by several vendors. It has become widely used to study ubiquitinated inclusions seen in Alzheimer’s and other kinds of disease. MCA-Ubi-1 was raised against purified ubiquitin conjugated with glutaraldehyde to keyhole limpet hemocyanin. The clone was initially screened on ELISA of the immunogen, and subsequently tested on sections of Alzheimer brain. MCA-Ubi-1 was one of several clones which stained neurofibrillary tangles in frozen sectioned material strongly and specifically. Subsequent studies indicated that MCA-Ubi-1 is relatively insensitive to formalin fixation and so can be used on mildly fixed histological sections of human brain for studies of Alzheimer’s disease. The antibody also works on paraffin embedded material. MCA-Ubi-1 also recognizes other ubiquitinated inclusion bodies such as the Lewy bodies of Parkinson’s disease and the Pick bodies in Pick’s disease in formalin fixed tissues. MCA-Ubi-1 will recognize human, bovine, chicken, Drosophila, and C. elegans in ELISA. MCA-Ubi-1 also works on Western blots and can be used to study ubiquitinated proteins. It has also been successfully used in competitive ELISA. MCA-Ubi-1 is a mouse IgG1 with a k light chain.
Synonyms: FLJ25987 Antibody
MGC8385 Antibody
Polyubiquitin B Antibody
RPS 27A Antibody
RPS27A Antibody
UBA 52 Antibody
UBA 80 Antibody
UBA52 Antibody
UBA80 Antibody
UBB Antibody
UBB_HUMAN Antibody
UBC Antibody
UBCEP 1 Antibody
UBCEP 2 Antibody
UBCEP1 Antibody
UBCEP2 Antibody
Ubiquitin B Antibody
Immunohistochemisty - Anti-Ubiquitin Antibody from EnCor Biotechnology (MCA-Ubi-1) - Antibodies.com
MCA-Ubi-1 staining of cerebral cortex of an Alzheimer patient. Neurofibrillary tangles and dystrophic neurites associated with senile plaques stain strongly with this antibody. In the center is a typical neurofibrillary tangle containing neuron.
Western Blot - Anti-Ubiquitin Antibody from EnCor Biotechnology (MCA-Ubi-1) - Antibodies.com
Blots of mono and polyubiquitin (lane 1), monoubiquitin only (2), and 100 μg total wet weight of cerebellum , cortex and brain stem respectively (lane 3-5). Material was run out on 20% SDS-PAGE and transferred electrophoretically to PVDF.
Please Note: All batches are extensively validated and are guaranteed to work as specified, however, QC data is not necessarily from the current lot on sale.

Analysis of mutant SOD1 electrophoretic mobility by Blue Native gel electrophoresis; evidence for soluble multimeric assemblies.

   Please Note: These publications cite this antibody under different brand names & catalogue numbers.


Cites ab7254

Tikhmyanova N  et al. Small molecule perturbation of the CAND1-Cullin1-ubiquitin cycle stabilizes p53 and triggers Epstein-Barr virus reactivation. PLoS Pathog 13:e1006517 (2017).

Cites ab7254

Jiang YF  et al. Electron tomographic analysis reveals ultrastructural features of mitochondrial cristae architecture which reflect energetic state and aging. Sci Rep 7:45474 (2017).

Cites ab7254

Wang J  et al. Tissue-Specific Ubiquitination by IPA1 INTERACTING PROTEIN1 Modulates IPA1 Protein Levels to Regulate Plant Architecture in Rice. Plant Cell 29:697-707 (2017).

Cites ab7254

Chen M  et al. APP modulates KCC2 expression and function in hippocampal GABAergic inhibition. Elife 6:N/A (2017).

Cites ab7254

Luo C  et al. PARAQUAT TOLERANCE3 Is an E3 Ligase That Switches off Activated Oxidative Response by Targeting Histone-Modifying PROTEIN METHYLTRANSFERASE4b. PLoS Genet 12:e1006332 (2016).

Cites ab7254

Qu Y  et al. Axitinib blocks Wnt/ß-catenin signaling and directs asymmetric cell division in cancer. Proc Natl Acad Sci U S A 113:9339-44 (2016).

Cites ab7254

Li Q  et al. Temporal Profiles of Stress Protein Inductions after Focal Transient Ischemia in Mice Brain. J Stroke Cerebrovasc Dis 25:2344-51 (2016).

Cites ab7254

Yu Z  et al. Lys29-linkage of ASK1 by Skp1-Cullin 1-Fbxo21 ubiquitin ligase complex is required for antiviral innate response. Elife 5:N/A (2016).

Cites ab7254

Farrawell NE  et al. Distinct partitioning of ALS associated TDP-43, FUS and SOD1 mutants into cellular inclusions. Sci Rep 5:13416 (2015).

Cites ab7254

Schumacher FR  et al. Characterisation of the Cullin-3 mutation that causes a severe form of familial hypertension and hyperkalaemia. EMBO Mol Med 7:1285-306 (2015).

Cites ab7254

Gatliff J  et al. TSPO interacts with VDAC1 and triggers a ROS-mediated inhibition of mitochondrial quality control. Autophagy 10:2279-96 (2014).

Cites ab7254

Liu C  et al. Null and hypomorph Prickle1 alleles in mice phenocopy human Robinow syndrome and disrupt signaling downstream of Wnt5a. Biol Open 3:861-70 (2014).

Cites ab7254

Bounedjah O  et al. Free mRNA in excess upon polysome dissociation is a scaffold for protein multimerization to form stress granules. Nucleic Acids Res 42:8678-91 (2014).

Cites ab7254

Pla A  et al. TLR4 mediates the impairment of ubiquitin-proteasome and autophagy-lysosome pathways induced by ethanol treatment in brain. Cell Death Dis 5:e1066 (2014).

Cites ab7254

Andersson V  et al. Enhancing protein disaggregation restores proteasome activity in aged cells. Aging (Albany NY) 5:802-12 (2013).

Cites ab7254

Zhang D  et al. Acetylation regulates Jun protein turnover in Drosophila. Biochim Biophys Acta 1829:1218-1224 (2013).

Cites ab7254

Jin X  et al. A potential role for CHH DNA methylation in cotton fiber growth patterns. PLoS One 8:e60547 (2013).

Cites ab7254

Nunes P  et al. Hypertonic stress promotes autophagy and microtubule-dependent autophagosomal clusters. Autophagy 9:550-67 (2013).

Cites ab7254

Khoronenkova SV & Dianov GL USP7S-dependent inactivation of Mule regulates DNA damage signalling and repair. Nucleic Acids Res 41:1750-6 (2013).

Cites ab7254

Ye S  et al. The E3 Ubiquitin Ligase Neuregulin Receptor Degradation Protein 1 (Nrdp1) Promotes M2 Macrophage Polarization by Ubiquitinating and Activating Transcription Factor CCAAT/Enhancer-binding Protein ß (C/EBPß). J Biol Chem 287:26740-8 (2012).

Cites ab7254

Ohmi K  et al. Defects in the medial entorhinal cortex and dentate gyrus in the mouse model of Sanfilippo syndrome type B. PLoS One 6:e27461 (2011).

Cites ab7254

Castiel A  et al. The Stil protein regulates centrosome integrity and mitosis through suppression of Chfr. J Cell Sci 124:532-9 (2011).

Cites ab7254

Tannous P  et al. Autophagy is an adaptive response in desmin-related cardiomyopathy. Proc Natl Acad Sci U S A 105:9745-50 (2008).

Cites ab7254

Tannous P  et al. Intracellular protein aggregation is a proximal trigger of cardiomyocyte autophagy. Circulation 117:3070-8 (2008).

Cites ab7254

Hasegawa M  et al. A critical role of RICK/RIP2 polyubiquitination in Nod-induced NF-kappaB activation. EMBO J 27:373-83 (2008).

Cites ab7254

Kuo CT  et al. Dendrite-specific remodeling of Drosophila sensory neurons requires matrix metalloproteases, ubiquitin-proteasome, and ecdysone signaling. Proc Natl Acad Sci U S A 102:15230-5 (2005).

Cites ab7254

Woods YL  et al. p14 Arf promotes small ubiquitin-like modifier conjugation of Werners helicase. J Biol Chem 279:50157-66 (2004).

Cites ab7254

Boutajangout A  et al. Characterisation of cytoskeletal abnormalities in mice transgenic for wild-type human tau and familial Alzheimer's disease mutants of APP and presenilin-1. Neurobiol Dis 15:47-60 (2004).

Cites ab7254

Wang DS  et al. Contribution of changes in ubiquitin and myelin basic protein to age-related cognitive decline. Neurosci Res 48:93-100 (2004).

Cites ab7254

He CZ & Hays AP Expression of peripherin in ubiquinated inclusions of amyotrophic lateral sclerosis. J Neurol Sci 217:47-54 (2004).

Cites ab7254

Lichtenfels R  et al. Identification of metabolic enzymes in renal cell carcinoma utilizing PROTEOMEX analyses. Biochim Biophys Acta 1646:21-31 (2003).

Cites ab7254

Ungureanu D  et al. Regulation of Jak2 through the ubiquitin-proteasome pathway involves phosphorylation of Jak2 on Y1007 and interaction with SOCS-1. Mol Cell Biol 22:3316-26 (2002).

Fortun J, Go JC, Li J, Amici SA, Dunn WA Jr, Notterpek L. Alterations in degradative pathways and protein aggregation in a neuropathy model based on PMP22 overexpression. Neurobiol Dis. 22:153-164 2006

Boutajangout A, Authelet M, Blanchard V, Touchet N, Tremp G, Pradier L, Brion JP. Characterisation of cytoskeletal abnormalities in mice transgenic for wild-type human tau and familial Alzheimer’s disease mutants of APP and presenilin-1. Neurobiol Dis. 15:47-60 (2004).

Wang DS, Bennett DA, Mufson EJ, Mattila P, Cochran E, Dickson DW. Contribution of changes in ubiquitin and myelin basic protein to age-related cognitive decline. Neurosci. Res. 48:93-100 (2004)

He CZ, Hays AP. Expression of peripherin in ubiquinated inclusions of amyotrophic lateral sclerosis. J. Neurol. Sci. 217:47-54 (2004)

Ungureanu D, Saharinen P, Junttila I, Hilton DJ, Silvennoinen O. Regulation of Jak2 through the ubiquitin-proteasome pathway involves phosphorylation of Jak2 on Y1007 and interaction with SOCS-1. Mol Cell Biol. 22:3316-26 (2002)

Wirbelauer C, Sutterluty H, Blondel M, Gstaiger M, Peter M, Reymond F, Krek W. The F-box protein Skp2 is a ubiquitylation target of a Cul1-based core ubiquitin ligase complex: evidence for a role of Cul1 in the suppression of Skp2 expression in quiescent fibroblasts. EMBO J. 19:5362-75 (2000)

Harris KF, Shoji I, Cooper EM, Kumar S, Oda H, Howley PM. Ubiquitin-mediated degradation of active Src tyrosine kinase. Proc Natl Acad Sci U S A. 96:13738-43 (1999)

Marti A, Wirbelauer C, Scheffner M, Krek W. Interaction between ubiquitin-protein ligase SCFSKP2 and E2F-1 underlies the regulation of E2F-1 degradation. Nat Cell Biol. 1:14-9 (1999)

Sternsdorf T, Puccetti E, Jensen K, Hoelzer D, Will H, Ottmann OG, Ruthardt M. PIC-1/SUMO-1-modified PML-retinoic acid receptor alpha mediates arsenic trioxide-induced apoptosis in acute promyelocytic leukemia. Mol Cell Biol. 19:5170-8 (1999)

Rating

Species

Application

Date

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Mouse

WB

11-Nov-2016

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Human

ICC/IF

26-Mar-2014

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Drosophila melanogaster

ICC/IF

11-Feb-2013

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Human

WB

13-Dec-2011

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Human

WB

23-Mar-2011

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Human

WB

13-Nov-2006

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13-Apr-2006


Description: Rabbit monoclonal antibody to Ubiquitin
Application: WB, ICC/IF, IHC, FC
Host: Rabbit
Reactivity: Human, Mouse, Rat
Immunogen: Recombinant antibody.

£269 – £400

Description: Rabbit monoclonal antibody to Ubiquitin.
Application: WB, IHC, IF, FC
Host: Rabbit
Isotype: IgG
Reactivity: Human, Mouse, Rat

£372 – £565

Total Citations (3)
Description: Rabbit polyclonal antibody to Ubiquitin (A46)
Application: WB, IHC
Host: Rabbit
Reactivity: Human, Mouse, Rat
Immunogen: Synthetic peptide, corresponding to amino acids 20-60 of Ubiquitin.

£165 – £283

Description: Rabbit polyclonal antibody to UBC.
Application: WB, IHC
Host: Rabbit
Isotype: IgG
Reactivity: Human, Mouse, Rat

£148 – £372

Please Note: Anti-Ubiquitin Antibody is for research use only. It is not intended for diagnostic or therapeutic use.
Antibodies.com | MCA-Ubi-1
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50µl £165
100µl £230
500µl £714

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