Anti-14-3-3 zeta Antibody (A10177)

Name: Anti-14-3-3 zeta Antibody
Brand: Antibodies.com
SKU: A10177
Price: 335.0 USD
Availability: InStock

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$335

Rabbit polyclonal antibody to 14-3-3 zeta for WB, IHC and ICC/IF.

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Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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Specifications

Name

Anti-14-3-3 zeta Antibody

Description

Rabbit polyclonal antibody to 14-3-3 zeta.

Applications

WB, IHC, ICC/IF

Dilutions

WB: 1:500-1:2,000, IHC: 1:50-1:200, ICC/IF: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 190 to the C-terminus of human YWHAZ (NP_003397.1).

Sequence

SLAKTAFDEAIAELDTLSEESYKDSTLIMQLLRDNLTLWTSDTQGDEAEAGEGGEN

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

28 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.01% Thiomersal.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

14 3 3 delta, 14 3 3 protein zeta/delta, 14 3 3 protein/cytosolic phospholipase A2, 14 3 3 zeta, 14-3-3 protein zeta/delta, 1433Z_HUMAN, Epididymis luminal protein 4, Epididymis secretory protein Li 3, HEL S 3, HEL4, KCIP-1, KCIP1, MGC111427, MGC126532, MGC138156, Phospholipase A2, Protein kinase C inhibitor protein 1, Tyrosine 3 monooxygenase/tryptophan 5 monooxygenase activation protein, delta polypeptide, Tyrosine 3 monooxygenase/tryptophan 5 monooxygenase activation protein, zeta, Tyrosine 3 monooxygenase/tryptophan 5 monooxygenase activation protein, zeta polypeptide, Tyrosine 3/tryptophan 5 monooxygenase activation protein, zeta polypeptide, YWHAD, YWHAZ

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (3)

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Figure 1: Western Blot - Anti-14-3-3 zeta Antibody (A10177)

Western blot analysis of extracts of various cell lines, using Anti-14-3-3 zeta Antibody (A10177) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 30s.

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Figure 2: Immunohistochemistry - Anti-14-3-3 zeta Antibody (A10177)

Immunohistochemistry analysis of paraffin-embedded rat kidney using Anti-14-3-3 zeta Antibody (A10177) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

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Figure 3: Immunofluorescence - Anti-14-3-3 zeta Antibody (A10177)

Immunofluorescence analysis of rat brain using Anti-14-3-3 zeta Antibody (A10177) at a dilution of 1:100. DAPI was used to stain the cell nuclei (blue).

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