Anti-160 kD Neurofilament Medium Antibody [ARC0396] (A305755)

Name: Anti-160 kD Neurofilament Medium Antibody [ARC0396]
Brand: Antibodies.com
SKU: A305755
Price: 470.0 USD
Availability: InStock

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$470

Rabbit monoclonal [ARC0396] antibody to 160 kD Neurofilament Medium for WB and ICC/IF.

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Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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Neuronal Marker

Specifications

Name

Anti-160 kD Neurofilament Medium Antibody [ARC0396]

Description

Rabbit monoclonal [ARC0396] antibody to 160 kD Neurofilament Medium.

Applications

WB, ICC/IF

Dilutions

WB: 1:500-1:2,000, ICC/IF: 1:50-1:200

Reactivity

Human

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 600-700 of human Neurofilament M (P07197).

Sequence

ELVADAKVEKPEKAKSPVPKSPVEEKGKSPVPKSPVEEKGKSPVPKSPVEEKGKSPVPKSPVEEKGKSPVSKSPVEEKAKSPVPKSPVEEAKSKAEVGKGE

Host

Rabbit

Clonality

Monoclonal

Clone ID

ARC0396

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

160 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

150kDa medium, NEF3, NEFM, Neurofilament 3, NF160, NFM

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (2)

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Figure 1: Western Blot - Anti-160 kD Neurofilament Medium Antibody [ARC0396] (A305755)

Western blot analysis of extracts of various cell lines, using Anti-160 kD Neurofilament Medium Antibody [ARC0396] (A305755) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 1s.

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Figure 2: Immunofluorescence - Anti-160 kD Neurofilament Medium Antibody [ARC0396] (A305755)

Immunofluorescence analysis of SH-SY5Y cells using Anti-160 kD Neurofilament Medium Antibody [ARC0396] (A305755) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).

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