Principle of Assay
The principle of this 30 Minute IHC DAB Detection System is that Linker 1 introduces HRP into the system, Linker 2 introduces Biotin into the system, and Tracer introduces Streptavidin and additional HRP into the system. This 30 Minute IHC DAB Detection System is an enzyme mediated reaction that utilizes horseradish peroxidase (HRP) to catalyze the deposition of two separate Linkers applied sequentially onto tissue sections or cell preparations. The deposited Linkers can be detected with a Streptavidin Peroxidase conjugate followed by a reaction with a peroxidase substrate/chromogen solution such as diaminobenzidine (DAB).
