Figure 1: Western Blot - Anti-AADACL1 Antibody (A121150)
Anti-EDF1 Antibody (A121147) (0.5µg/ml) staining of HEK293 (A), HeLa (B) and (1µg/ml) NIH3T3 (C) Nuclear cell lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
Anti-EDF1 Antibody (A121147) - Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10µg/ml) followed by Alexa Fluor 488 secondary antibody (2µg/ml), showing nuclear staining. Actin filaments were stained with phalloidin (red) and the nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10µg/ml) followed by Alexa Fluor 488 secondary antibody (2µg/ml).