Anti-ACSL3 Antibody [ARC55219] (A305357)

Name: Anti-ACSL3 Antibody [ARC55219]
Brand: Antibodies.com
SKU: A305357

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This product is discontinued

Anti-ACSL3 Antibody [ARC55219] (A305357) has been discontinued and is no longer available.

View all Anti-ACSL3 Antibodies.

Specifications

Name

Anti-ACSL3 Antibody [ARC55219]

Description

Rabbit monoclonal [ARC55219] antibody to ACSL3.

Applications

WB, ICC/IF

Dilutions

WB: 1:10,000-1:30,000, ICC/IF: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 600-700 of human ACSL3 (NP_004448.2).

Sequence

ALKNLPLVDNICAYANSYHSYVIGFVVPNQKELTELARKKGLKGTWEELCNSCEMENEVLKVLSEAAISASLEKFEIPVKIRLSPEPWTPETGLVTDAFKL

Host

Rabbit

Clonality

Monoclonal

Clone ID

ARC55219

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

75 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.05% Proclin 300.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

ACS3, ACSL3_HUMAN, Acyl CoA synthetase long chain family member 3, FACL3, Fatty acid Coenzyme A ligase long chain 3, LACS 3, LACS3, Lignoceroyl CoA synthase, Long chain acyl CoA synthetase 3, Long chain fatty acid CoA ligase 3, Long-chain acyl-CoA synthetase 3, Long-chain-fatty-acid--CoA ligase 3, PRO2194

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (3)

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Figure 1: Western Blot - Anti-ACSL3 Antibody [ARC55219] (A305357)

Western blot analysis of extracts of various cell lines, using Anti-ACSL3 Antibody [ARC55219] (A305357) at 1:30,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 180s.

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Figure 2: Western Blot - Anti-ACSL3 Antibody [ARC55219] (A305357)

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Figure 3: Immunofluorescence - Anti-ACSL3 Antibody [ARC55219] (A305357)

Immunofluorescence analysis of K-562 using Anti-ACSL3 Antibody [ARC55219] (A305357) at a dilution of 1:100(40x lens). DAPI was used to stain the cell nuclei (blue).

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