Activated R-Phycoerythrin (R-PE) (A269983)

$315
Freight Charges
Lead Time
7-8 Business Days
Telephone
+1 (314) 370-6046
Mon - Fri, 8am - 4pm AST
Email
orders@antibodies.com
Name
Activated R-Phycoerythrin (R-PE)
Description
Activated R-Phycoerythrin (R-PE) is an intensely bright phycobiliprotein isolated from newly identified red algae species exclusive to the coastal region of Taiwan. It exhibits extremely bright red-orange fluorescence with high quantum yields, and generates fluorescence signals far stronger than other commonly-seen organic and synthetic fluorophores. Activated R-PE has a maleimide group introduced, which allows it to be conveniently conjugated to the thiol groups of proteins, without the need for additional activation and purification. This facilitates the rapid conjugation of PE to antibodies, other proteins, and reagents.
Applications
Flow Cytometry, IF, ELISA, Microarray
Molecular Weight
240 kDa
Concentration
9.5 - 10.5 mg/ml
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH7.5.
Storage
Store in the dark at 2-8°C. Avoid prolonged exposure to light. Do not freeze!
General Notes

With a broad range of excitation wavelengths, R-PE can be easily excited by two laser sources (488 nm & 561 nm), and used in most fluorescence detection applications, e.g. Flow Cytometry (FC), Immunofluorescence microscopy (IF), and Microarrays. Home Algae's R-PE absorbance maxima are at 496, 546, and 565 nm, with a resolvable emission peak at 578 nm. It shows minimal fluoresence quenching.

This formulation is a ready-to-use reagent, with a meleimide group attached for simple conjugation to protein thiol groups, and is free from Ammonium Sulfate.

R-PE won't penetrate into the cell and cause a false-positive reaction.

R-PE has multiple sites for stable conjugation with Biotin, Streptavidin, and other fluorophores, e.g., Cy-3, Cy-5, Cy-7.

Non-toxic, with less chance of affecting characteristics of the sample, human body, and environment.

Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
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